Development of Biodegradable Polycation-Based Inhalable Dry Gene Powders by Spray Freeze Drying

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2015 Sep 8

PMID 26343708

Citations 11

Authors

Tomoyuki Okuda

Yumiko Suzuki

Yuko Kobayashi

Takehiko Ishii

Satoshi Uchida

Keiji Itaka

Kazunori Kataoka

Hirokazu Okamoto

Affiliations

Soon will be listed here.

Abstract

In this study, two types of biodegradable polycation (PAsp(DET) homopolymer and PEG-PAsp(DET) copolymer) were applied as vectors for inhalable dry gene powders prepared by spray freeze drying (SFD). The prepared dry gene powders had spherical and porous structures with a 5~10-μm diameter, and the integrity of plasmid DNA could be maintained during powder production. Furthermore, it was clarified that PEG-PAsp(DET)-based dry gene powder could more sufficiently maintain both the physicochemical properties and in vitro gene transfection efficiencies of polyplexes reconstituted after powder production than PAsp(DET)-based dry gene powder. From an in vitro inhalation study using an Andersen cascade impactor, it was demonstrated that the addition of l-leucine could markedly improve the inhalation performance of dry powders prepared by SFD. Following pulmonary delivery to mice, both PAsp(DET)- and PEG-PAsp(DET)-based dry gene powders could achieve higher gene transfection efficiencies in the lungs compared with a chitosan-based dry gene powder previously reported by us.

Citing Articles

Stability of Naked Nucleic Acids under Physical Treatment and Powder Formation: Suitability for Development as Dry Powder Formulations for Inhalation.

Okuda T, Okazaki M, Hayano A, Okamoto H Pharmaceutics. 2023; 15(12).

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Engineering Inhalable Therapeutic Particles: Conventional and Emerging Approaches.

Negi A, Nimbkar S, Moses J Pharmaceutics. 2023; 15(12).

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Aerosolizable Plasmid DNA Dry Powders Engineered by Thin-film Freezing.

Xu H, Moon C, Sahakijpijarn S, Dao H, Alzhrani R, Wang J Pharm Res. 2023; 40(5):1141-1152.

PMID: 36703028 PMC: 9879621. DOI: 10.1007/s11095-023-03473-5.

Spray Freeze Drying of Biologics: A Review and Applications for Inhalation Delivery.

Farinha S, Sa J, Lino P, Galesio M, Pires J, Rodrigues M Pharm Res. 2022; 40(5):1115-1140.

PMID: 36456666 DOI: 10.1007/s11095-022-03442-4.

Dry Powder for Pulmonary Delivery: A Comprehensive Review.

Chaurasiya B, Zhao Y Pharmaceutics. 2020; 13(1).

PMID: 33379136 PMC: 7824629. DOI: 10.3390/pharmaceutics13010031.

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