Evaluation of Iron Deficiency Using Reticulocyte Indices in Dogs Enrolled in a Blood Donor Program

Overview

Journal J Vet Intern Med

Specialties General Medicine
Veterinary Medicine

Date 2015 Sep 5

PMID 26340143

Citations 1

Authors

D S Foy

K R Friedrichs

J F Bach

Affiliations

Soon will be listed here.

Abstract

Background: People donating blood more than twice annually are at risk of developing iron deficiency. Little is known about the iron status of dogs enrolled in blood donor programs.

Hypothesis: Dogs donating blood ≥6 times annually will show evidence of iron deficiency based on their reticulocyte indices.

Animals: Thirteen dogs enrolled in a blood donor program donating ≥6 times over the preceding 12 months and 20 healthy nondonor control dogs.

Methods: Prospective observational study. Mature red blood cell (RBC) indices, reticulocyte indices, serum iron, serum ferritin, and total iron-binding capacity (TIBC) were compared between groups.

Results: Packed cell volume (median 47%, range 40-52%, P < .01), hematocrit (median 46.4%, range 40.3-52.5%, P < .01), and reticulocyte count (median 16,000/μL, range 9,000-38,000/μL, P < .01) were significantly lower in the blood donor dogs. No statistically significant differences were noted in the mature RBC indices between groups. Both reticulocyte mean corpuscular volume (median 88.8 fL, range 83.4-95.5 fL, P = .03) and reticulocyte hemoglobin content (median 24.6 pg, range 23.1-26.6 pg, P < .01) were significantly lower in the blood donor group. Serum iron and ferritin were similar between groups; however, TIBC was significantly higher in the control group (median 403 μg/dL, range 225-493 μg/dL, P = .02).

Conclusions: The findings in dogs donating ≥6 times annually suggest the presence of iron-deficient erythropoiesis in this population.

Citing Articles

Evaluation of an intramuscular butorphanol and alfaxalone protocol for feline blood donation: a pilot study.

Granfone M, Walker J, Smith L J Feline Med Surg. 2017; 20(8):793-798.

PMID: 28948901 PMC: 11104132. DOI: 10.1177/1098612X17732484.

References

Ferreira R, Gopegui R, Araujo M, de Matos A . Effects of repeated blood donations on iron status and hematologic variables of canine blood donors. J Am Vet Med Assoc. 2014; 244(11):1298-303. DOI: 10.2460/javma.244.11.1298. View

Radtke H, Meyer T, Kalus U, Rocker L, Salama A, Kiesewetter H . Rapid identification of iron deficiency in blood donors with red cell indexes provided by Advia 120. Transfusion. 2005; 45(1):5-10. DOI: 10.1111/j.1537-2995.2005.04205.x. View

Finch C, Cook J, LABBE R, Culala M . Effect of blood donation on iron stores as evaluated by serum ferritin. Blood. 1977; 50(3):441-7. View

Naigamwalla D, Webb J, Giger U . Iron deficiency anemia. Can Vet J. 2012; 53(3):250-6. PMC: 3280776. View

Farrugia A . Iron and blood donation- an under-recognised safety issue. Dev Biol (Basel). 2007; 127:137-46. View

Agarwal R . Nonhematological benefits of iron. Am J Nephrol. 2007; 27(6):565-71. DOI: 10.1159/000107927. View

Skikne B, Lynch S, Borek D, Cook J . Iron and blood donation. Clin Haematol. 1984; 13(1):271-87. View

Kotisaari S, Romppanen J, Penttila I, Punnonen K . The Advia 120 red blood cells and reticulocyte indices are useful in diagnosis of iron-deficiency anemia. Eur J Haematol. 2002; 68(3):150-6. DOI: 10.1034/j.1600-0609.2002.01532.x. View

Eden A . Iron deficiency and impaired cognition in toddlers: an underestimated and undertreated problem. Paediatr Drugs. 2005; 7(6):347-52. DOI: 10.2165/00148581-200507060-00003. View

10.

Simon T, Garry P, Hooper E . Iron stores in blood donors. JAMA. 1981; 245(20):2038-43. View

11.

Zaldivar-Lopez S, Iazbik M, Marin L, Couto C . Iron status in blood donor dogs. J Vet Intern Med. 2014; 28(1):211-4. PMC: 4895534. DOI: 10.1111/jvim.12254. View

12.

Bohn A . Diagnosis of disorders of iron metabolism in dogs and cats. Vet Clin North Am Small Anim Pract. 2013; 43(6):1319-30, vii. DOI: 10.1016/j.cvsm.2013.07.002. View

13.

Lippi G, Salvagno G, Montagnana M, Brocco G, Guidi G . Influence of hemolysis on routine clinical chemistry testing. Clin Chem Lab Med. 2006; 44(3):311-6. DOI: 10.1515/CCLM.2006.054. View

14.

Degen M . Pseudohyperkalemia in Akitas. J Am Vet Med Assoc. 1987; 190(5):541-3. View

15.

Cable R, Glynn S, Kiss J, Mast A, Steele W, Murphy E . Iron deficiency in blood donors: analysis of enrollment data from the REDS-II Donor Iron Status Evaluation (RISE) study. Transfusion. 2010; 51(3):511-22. PMC: 3050998. DOI: 10.1111/j.1537-2995.2010.02865.x. View

16.

Potkay S, ZINN R . Effects of collection interval, body weight, and season on the hemograms of canine blood donors. Lab Anim Care. 1969; 19(2):192-8. View

17.

Bowlus C . The role of iron in T cell development and autoimmunity. Autoimmun Rev. 2003; 2(2):73-8. DOI: 10.1016/s1568-9972(02)00143-x. View

18.

Kiss J, Steele W, Wright D, Mast A, Carey P, Murphy E . Laboratory variables for assessing iron deficiency in REDS-II Iron Status Evaluation (RISE) blood donors. Transfusion. 2013; 53(11):2766-75. PMC: 3895107. DOI: 10.1111/trf.12209. View

19.

Yamato O, Lee K, Chang H, Tajima M, Maede Y . Relation between erythrocyte reduced glutathione and glutamate concentrations in Korean Jindo dogs with erythrocytes possessing hereditary high activity of Na-K-ATPase and a high concentration of potassium. J Vet Med Sci. 1999; 61(10):1179-82. DOI: 10.1292/jvms.61.1179. View

20.

Bhandari S, Norfolk D, Brownjohn A, Turney J . Evaluation of RBC ferritin and reticulocyte measurements in monitoring response to intravenous iron therapy. Am J Kidney Dis. 1997; 30(6):814-21. DOI: 10.1016/s0272-6386(97)90087-1. View