Effect of Lactobacillus Salivarius on Th1/Th2 Cytokines and the Number of Spleen CD4⁺ CD25⁺ Foxp3⁺ Treg in Asthma Balb/c Mouse

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2015 Sep 5

PMID 26339333

Citations 13

Authors

Xiang Yun

Yunxiao Shang

Miao Li

Affiliations

Soon will be listed here.

Abstract

Background: Bronchial asthma is a chronic airway inflammatory disease that involves T lymphocytes.

Methods: In order to explore the effect of Lactobacillus salivarius on Th1/Th2 cytokines and the number of spleen CD4(+) CD25(+) Foxp3(+) Treg in asthma Balb/c mouse, we constructed acute asthma model with ovalbumin to observe the mouse behavior change in Balb/c mice. The expression of GATA-3 mRNA and T-bet mRNA was measured by real-time PCR. The proportion of CD4(+) CD25(+) Foxp3(+) Treg/CD4(+) was determined by flow cytometry.

Results: The results demonstrated that oral gavage with Lactobacillus salivarius before sensitization could alleviate the clinical symptoms, airway hyper-reactivity and airway inflammation in asthma mouse to some extent; Lactobacillus salivarius may improve the imbalance of Th1/Th2 in asthma mouse through increasing the expression of T-bet mRNA at the transcriptional level and inhibiting the expression of GATA-3 mRNA simultaneously.

Conclusion: CD4(+) CD25(+) Foxp3(+) Treg cells may be involved in the pathogenesis of bronchial asthma, and may be the upstream regulatory mechanism of the improvement of Th1/Th2 imbalance by Lactobacillus salivarius.

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