A Comparison of MicroCT and MicroPET for Evaluating Lymph Node Metastasis in a Rat Model

Overview

Journal Mol Imaging Biol

Publisher Springer

Specialties Molecular Biology
Radiology

Date 2015 Sep 3

PMID 26330160

Citations 2

Authors

Paul Flechsig

Clemens Kratochwil

Arne Warth

Daniel Rath

Viktoria Eichwald

Peter E Huber

Hans-Ulrich Kauczor

Uwe Haberkorn

Frederik L Giesel

Affiliations

Soon will be listed here.

Abstract

Purpose: The demand to optimize multidisciplinary treatment strategies in patients with benign and malignant diseases of the lung and other organs has led to the increased need of mechanistic proof-of-concept studies in preclinical small animal models using new non-invasive imaging methods. Therefore, we evaluated the role of microPET and microCT for mediastinal lymph node staging in an orthotopic lung cancer model in rats.

Procedures: Human lung cancer cells (NCI-H460) were injected transthoracically in nude rats (NIH-RNU). After 2 weeks of tumour growth, animals underwent multiphase contrast-enhanced microCT using ExiTron nano 12000 as a contrast agent and dynamic microPET using the tracer 2-deoxy-2-[(18)F]fluoro-D-glucose ([(18)F]FDG). Thereafter, animals were sacrificed for histological analysis.

Results: Late phase micro X-ray computed tomography (microCT) revealed the best delineation of lymph node metastases, as compared to earlier scans. In terms of an increased [(18)F]FDG uptake over time, dynamic micro positron emission tomography (microPET) delineated lymph node metastases and enabled metabolic examinations of the induced lung cancer metastases.

Conclusion: The combination of contrast-enhanced microCT and dynamic microPET is feasible in rats for the visualization of mediastinal lymph node metastases.

Citing Articles

The Value of Micro-CT in the Diagnosis of Lung Carcinoma: A Radio-Histopathological Perspective.

Dizbay Sak S, Sevim S, Buyuksungur A, Kayi Cangir A, Orhan K Diagnostics (Basel). 2023; 13(20).

PMID: 37892083 PMC: 10606474. DOI: 10.3390/diagnostics13203262.

Lung ultrasound as a monitoring tool in lung transplantation in rodents: a feasibility study.

Diana P, Zampieri D, Furlani E, Pivetta E, Calabrese F, Pezzuto F J Thorac Dis. 2018; 10(7):4274-4282.

PMID: 30174873 PMC: 6106042. DOI: 10.21037/jtd.2018.06.52.

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