Adenovirus-mediated Interleukin-35 Gene Transfer Suppresses Allergic Airway Inflammation in a Murine Model of Asthma

Overview

Journal Inflamm Res

Specialties Allergy & Immunology
Pathology

Date 2015 Aug 31

PMID 26318911

Citations 18

Authors

Yan Li

Xiuhe Pan

Xiao Peng

Shubo Li

Yanchun Zhou

Xiaoxuan Zheng

Mingcai Li

Affiliations

Soon will be listed here.

Abstract

Objective And Design: Asthma is thought to result from the generation of T helper type 2 (Th2) responses, leading to bronchial inflammation. Interleukin (IL)-35 is a recently described member of IL-12 cytokine family that plays a critical role in influencing Th cell differentiation and inflammatory processes. The aim of this study was to examine the effect of adenovirus expressing IL-35 (AdIL-35) on allergic airway hyperresponsiveness (AHR) and inflammation in a mouse model of asthma.

Methods: BALB/c mice were subjected to an established model of allergic airway disease. AdIL-35 was administered intranasally and the effect of IL-35 on Th2 responses, pulmonary inflammation, goblet cell metaplasia, and AHR were assessed.

Results: Transfer of AdIL-35 significantly reduced the severity of AHR and numbers of inflammatory cells and levels of IL-4, IL-5, IL-13, and IL-17 in bronchoalveolar lavage fluid, compared with administration of a control virus. Moreover, AdIL-35 elevated the numbers of CD4+CD25+Foxp3+ regulatory T cells in the lungs. Histological analysis showed that AdIL-35 inhibited allergic lung tissue inflammation and mucus hypersecretion.

Conclusion: These results demonstrate that adenovirus-mediated delivery of interleukin-35 gene can mitigate allergic airway inflammation in experimental asthma and suggest that IL-35 may offer a novel therapeutic approach to treat allergic asthma.

Citing Articles

IL-35 promotes IL-35IL-10 Bregs and Conventional LAG3 Tregs in the lung tissue of OVA-Induced Asthmatic Mice.

Saheb Sharif-Askari F, Zakri A, Alenazy M, El-Wetidy M, Khalid Salah Al-Sheakly B, Sharif-Askari N Inflamm Res. 2024; 73(10):1699-1709.

PMID: 39127869 DOI: 10.1007/s00011-024-01925-1.

Interplay of IL-33 and IL-35 Modulates Th2/Th17 Responses in Cigarette Smoke Exposure HDM-Induced Asthma.

Liu J, Su B, Tao P, Yang X, Zheng L, Lin Y Inflammation. 2023; 47(1):173-190.

PMID: 37737467 DOI: 10.1007/s10753-023-01902-6.

IL35 attenuated LPS-induced acute lung injury by regulating macrophage polarization.

Chen S, Xia J, Zhang Y, Zhan Q Mol Biol Rep. 2022; 49(7):5811-5820.

PMID: 35748972 PMC: 9244303. DOI: 10.1007/s11033-022-07293-5.

Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis.

Kouzaki H, Arai Y, Nakamura K, Murao T, Tojima I, Shimizu S Asia Pac Allergy. 2021; 11(3):e34.

PMID: 34386410 PMC: 8331259. DOI: 10.5415/apallergy.2021.11.e34.

Immunomodulatory cytokine interleukin-35 and immune thrombocytopaenia.

Zhu J, Shan N J Int Med Res. 2020; 48(12):300060520976477.

PMID: 33356722 PMC: 7768574. DOI: 10.1177/0300060520976477.

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