Advances in Boerhaavia Diffusa Hairy Root Technology: a Valuable Pursuit for Identifying Strain Sensitivity and Up-scaling Factors to Refine Metabolite Yield and Bioactivity Potentials

Overview

Journal Protoplasma

Publisher Springer

Specialty Biology

Date 2015 Aug 29

PMID 26315820

Citations 4

Authors

Ruby Gupta

Pallavi Pandey

Sailendra Singh

Dhananjay Kumar Singh

Archana Saxena

Suaib Luqman

Dnyaneshwar U Bawankule

Suchitra Banerjee

Affiliations

Soon will be listed here.

Abstract

The present study reports the Agrobacterium rhizogenes-mediated hairy root induction in of an ethno-medicinally significant herb-Boerhaavia diffusa L., for elucidating the underlying competence regarding its biosynthetic (i.e. boeravinone B and eupalitin) and bioactivity (antibacterial, antioxidant and anti-inflammatory) potentials. Host plant-specific receptiveness towards A. rhizogenes strains and disparity in compatibility threshold of leaf and nodal explants were evident. Only leaf explants responded, attaining hairy root induction with the ATCC 15834 followed by A4 and SA79 strains in reducing order of transformation efficiency. The growth behaviours differed amongst independent rhizoclones, and two clones of A4 (RBH) and ATCC 15834 (RBT8) origin demonstrated higher growth potentials. Polymerase chain reaction amplification of rol genes confirmed their transformed nature. Optimization of the appropriate solvent and reverse phase high-performance liquid chromatography parameters relating to the targeted metabolite production in the selected RBH and RBT8 clones revealed higher accumulation of eupalitin with the RBH clone having the best result of 1.44 times greater yield over the control root. Compared to the selected rhizoclones, the control roots however showed higher boeravinone B content. Devising a modified "stirred-tank" reactor through equipping with marine impellers and ring spargers facilitated high-density RBH root biomass yield with 6.1-fold and 1.15-fold yield increment of the boeravinone B and eupalitin respectively compared to shake-flask cultures. Considering the control roots, the RBH clone revealed analogous antioxidant/antibacterial activities with improved anti-inflammatory potential. The hairy root mediated higher production of boeravinone B and eupalitin could be achieved for the first time in bioreactor.

Citing Articles

The Untapped Potential of Hairy Root Cultures and Their Multiple Applications.

Mirmazloum I, Slavov A, Marchev A Int J Mol Sci. 2024; 25(23).

PMID: 39684394 PMC: 11641487. DOI: 10.3390/ijms252312682.

Effects of various Agrobacterium rhizogenes strains on hairy root induction and analyses of primary and secondary metabolites in Ocimum basilicum.

Sathasivam R, Choi M, Radhakrishnan R, Kwon H, Yoon J, Yang S Front Plant Sci. 2022; 13:983776.

PMID: 36325544 PMC: 9619037. DOI: 10.3389/fpls.2022.983776.

Hairy root culture technology: applications, constraints and prospect.

Gantait S, Mukherjee E Appl Microbiol Biotechnol. 2020; 105(1):35-53.

PMID: 33226470 DOI: 10.1007/s00253-020-11017-9.

Influence of different strains on hairy root induction and analysis of phenolic and flavonoid compounds in marshmallow ( L.).

Tavassoli P, Safipour Afshar A 3 Biotech. 2018; 8(8):351.

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