Involvement of Antizyme Characterized from the Small Abalone Haliotis Diversicolor in Gonadal Development

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Aug 28

PMID 26313647

Citations 3

Authors

Wei-Dong Li

Min Huang

Wen-Gang Lu

Xiao Chen

Ming-Hui Shen

Xiang-Min Li

Rong-Xia Wang

Cai-Huan Ke

Affiliations

Soon will be listed here.

Abstract

The small abalone Haliotis diversicolor is an economically important mollusk that is widely cultivated in Southern China. Gonad precocity may affect the aquaculture of small abalone. Polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. Ornithine decarboxylase (ODC) and antizyme (AZ) are essential elements of a feedback circuit that regulates cellular polyamines. This paper presents the molecular cloning and characterization of AZ from small abalone. Sequence analysis showed that the cDNA sequence of H. diversicolor AZ (HdiODCAZ) consisted of two overlapping open reading frames (ORFs) and conformed to the +1 frameshift property of the frame. Thin Layer chromatography (TLC) analysis suggested that the expressed protein encoded by +1 ORF2 was the functional AZ that targets ODC to 26S proteasome degradation. The result demonstrated that the expression level of AZ was higher than that of ODC in the ovary of small abalone. In addition, the expression profiles of ODC and AZ at the different development stages of the ovary indicated that these two genes might be involved in the gonadal development of small abalone.

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