Knockout of CGAS and STING Rescues Virus Infection of Plasmid DNA-Transfected Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2015 Aug 28

PMID 26311870

Citations 32

Authors

Martijn A Langereis

Huib H Rabouw

Melle Holwerda

Linda J Visser

Frank J M van Kuppeveld

Affiliations

Soon will be listed here.

Abstract

It is well known that plasmid DNA transfection, prior to virus infection, negatively affects infection efficiency. Here, we show that cytosolic plasmid DNA activates the cGAS/STING signaling pathway, which ultimately leads to the induction of an antiviral state of the cells. Using a transient one-plasmid clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system, we generated cGAS/STING-knockout cells and show that these cells can be infected after plasmid DNA transfection as efficiently as nontransfected cells.

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