Potato Virus Y HC-Pro Reduces the ATPase Activity of NtMinD, Which Results in Enlarged Chloroplasts in HC-Pro Transgenic Tobacco

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Aug 27

PMID 26309250

Citations 3

Authors

Yayi Tu

Zhenqian Zhang

Daofeng Li

Heng Li

Jiangli Dong

Tao Wang

Affiliations

Soon will be listed here.

Abstract

Potato virus Y (PVY) is an important plant virus and causes great losses every year. Viral infection often leads to abnormal chloroplasts. The first step of chloroplast division is the formation of FtsZ ring (Z-ring), and the placement of Z-ring is coordinated by the Min system in both bacteria and plants. In our lab, the helper-component proteinase (HC-Pro) of PVY was previously found to interact with the chloroplast division protein NtMinD through a yeast two-hybrid screening assay and a bimolecular fluorescence complementation (BiFC) assay in vivo. Here, we further investigated the biological significance of the NtMinD/HC-Pro interaction. We purified the NtMinD and HC-Pro proteins using a prokaryotic protein purification system and tested the effect of HC-Pro on the ATPase activity of NtMinD in vitro. We found that the ATPase activity of NtMinD was reduced in the presence of HC-Pro. In addition, another important chloroplast division related protein, NtMinE, was cloned from the cDNA of Nicotiana tabacum. And the NtMinD/NtMinE interaction site was mapped to the C-terminus of NtMinD, which overlaps the NtMinD/HC-Pro interaction site. Yeast three-hybrid assay demonstrated that HC-Pro competes with NtMinE for binding to NtMinD. HC-Pro was previously reported to accumulate in the chloroplasts of PVY-infected tobacco and we confirmed this result in our present work. The NtMinD/NtMinE interaction is very important in the regulation of chloroplast division. To demonstrate the influence of HC-Pro on chloroplast division, we generated HC-Pro transgenic tobacco with a transit peptide to retarget HC-Pro to the chloroplasts. The HC-Pro transgenic plants showed enlarged chloroplasts. Our present study demonstrated that the interaction between HC-Pro and NtMinD interfered with the function of NtMinD in chloroplast division, which results in enlarged chloroplasts in HC-Pro transgenic tobacco. The HC-Pro/NtMinD interaction may cause the formation of abnormal chloroplasts in PVY-infected plants.

Citing Articles

Potyviral Helper-Component Protease: Multifaced Functions and Interactions with Host Proteins.

Hyskova V, Belonoznikova K, Chmelik J, Hoffmeisterova H, cerovska N, Moravec T Plants (Basel). 2024; 13(9).

PMID: 38732454 PMC: 11085613. DOI: 10.3390/plants13091236.

The HCPro from the Potyviridae family: an enviable multitasking Helper Component that every virus would like to have.

Valli A, Gallo A, Rodamilans B, Lopez-Moya J, Garcia J Mol Plant Pathol. 2017; 19(3):744-763.

PMID: 28371183 PMC: 6638112. DOI: 10.1111/mpp.12553.

Chloroplast: the Trojan horse in plant-virus interaction.

Bhattacharyya D, Chakraborty S Mol Plant Pathol. 2017; 19(2):504-518.

PMID: 28056496 PMC: 6638057. DOI: 10.1111/mpp.12533.

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