Female Spontaneously Diabetic Torii Fatty Rats Develop Nonalcoholic Steatohepatitis-like Hepatic Lesions

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2015 Aug 21

PMID 26290633

Citations 7

Authors

Yukihito Ishii

Yu Motohashi

Makoto Muramatsu

Yoshiaki Katsuda

Katsuhiro Miyajima

Tomohiko Sasase

Takahisa Yamada

Tohru Matsui

Shinichi Kume

Takeshi Ohta

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the histological features of the liver in spontaneously diabetic Torii (SDT) fatty rats compared with age-matched Sprague-Dawley (SD) rats.

Methods: Female SDT Lepr(fa) (SDT fatty) rats and age-matched SD rats were fed ad libitum. Body weight and biochemical parameters, such as serum glucose, triglyceride (TG), total cholesterol (TC), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels as well as fatty acid and TG accumulation in the liver were evaluated at 8 wk of age in the non-fasting state and at 8-wk intervals from 8 to 40 wk of age. Histopathological examinations of the liver were performed using hematoxylin and eosin and Sirius Red staining as well as double staining for ED-1 and toluidine blue. The expression of genes involved in TG synthesis, inflammation, and fibrosis was examined in the liver.

Results: SDT fatty rats showed significantly increased body weight compared with SD rats. Serum glucose, TG, and TC levels were significantly higher in SDT fatty rats compared with SD rats. The serum AST and ALT levels in SDT fatty rats were significantly elevated at 8 wk of age compared with the levels in SD rats. Hepatic TG content was marked in SDT fatty rats from 8 to 32 wk of age. Histopathologically, severe hepatosteatosis accompanied by inflammation was observed at 8 wk of age, and fibrosis started to occur at 32 wk of age. Furthermore, Sirius Red and ED-1 staining were increased in the liver at 32 wk of age. Hepatic gene expression related to TG synthesis, inflammation and fibrosis tended to increase in SDT fatty rats compared with SD rats, and the gene expression related to TG secretion was decreased in SDT fatty rats compared with SD rats.

Conclusion: Female SDT fatty rats have the potential to become an important animal model of nonalcoholic steatohepatitis with type 2 diabetes and obesity.

Citing Articles

Pathophysiological Features of Rat Models of Nonalcoholic Fatty Liver Disease/Nonalcoholic Steatohepatitis.

Saigo Y, Uno K, Ishigure T, Odake T, Ohta T In Vivo. 2024; 38(3):990-999.

PMID: 38688597 PMC: 11059886. DOI: 10.21873/invivo.13532.

Characteristics of bile acid composition in high fat diet-induced nonalcoholic fatty liver disease in obese diabetic rats.

Nakade Y, Kitano R, Sakamoto K, Kimoto S, Yamauchi T, Inoue T PLoS One. 2021; 16(2):e0247303.

PMID: 33626072 PMC: 7904175. DOI: 10.1371/journal.pone.0247303.

Glomerular hyperfiltration with hyperglycemia in the spontaneously diabetic Torii (SDT) fatty rat, an obese type 2 diabetic model.

Sano R, Ishii Y, Yamanaka M, Yasui Y, Kemmochi Y, Kuroki F Physiol Res. 2021; 70(1):45-54.

PMID: 33453716 PMC: 8820518. DOI: 10.33549/physiolres.934533.

Teriparatide Improves Bone and Lipid Metabolism in a Male Rat Model of Type 2 Diabetes Mellitus.

Nomura S, Kitami A, Takao-Kawabata R, Takakura A, Nakatsugawa M, Kono R Endocrinology. 2019; 160(10):2339-2352.

PMID: 31504411 PMC: 6760306. DOI: 10.1210/en.2019-00239.

Pathophysiological abnormalities in the brains of Spontaneously Diabetic Torii-Lepr (SDT fatty) rats, a novel type 2 diabetic model.

Maekawa T, Ohta T, Kume S J Vet Med Sci. 2018; 80(9):1385-1391.

PMID: 30012919 PMC: 6160888. DOI: 10.1292/jvms.18-0296.

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