Fluorescent Mimics of Cholesterol That Rapidly Bind Surfaces of Living Mammalian Cells

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2015 Aug 20

PMID 26287483

Citations 2

Authors

David Hymel

Sutang Cai

Qi Sun

Rebecca S Henkhaus

Chamani Perera

Blake R Peterson

Affiliations

Soon will be listed here.

Abstract

Mammalian cells acquire cholesterol, a critical membrane constituent, through multiple mechanisms. We synthesized mimics of cholesterol, fluorescent N-alkyl-3β-cholesterylamine-glutamic acids, that are rapidly incorporated into cellular plasma membranes compared with analogous cholesteryl amides, ethers, esters, carbamates, and a sitosterol analogue. This process was inhibited by ezetimibe, indicating a receptor-mediated uptake pathway.

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