Multiple Inherited Thrombophilic Gene Polymorphisms in Spontaneous Abortions in Turkish Population

Overview

Journal Int J Mol Cell Med

Specialty Genetics

Date 2015 Aug 12

PMID 26261801

Citations 3

Authors

Sinem Yalcintepe

Ozturk Ozdemir

Servet Ozden Hacivelioglu

Cisem Akurut

Evrim Koc

Ahmet Uludag

Emine Cosar

Fatma Silan

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to investigate the possible role of multiple inherited thrombophilic gene variations in women with unexplained spontaneous abortions. For this purpose, the Factor V Leiden (FVL) (rs6025), Prothrombin G20210A (rs1799963), MTHFR C677T (rs1801133), PAI-1 4G/5G (rs1799889), ACE I/D (rs1799752), eNOS E298D (rs1799983), and Apo E E2/E3/E4 (rs429358) polymorphisms were genotyped and correlated in spontaneously aborted fetal materials, their mothers and fertile women. Twenty three abortion materials, 22 women with ≥1 unexplained fetal loss, and 22 control subjects with at least two healthy term infants as a control group were studied. Target SNPs for each gene were analyzed by real time-PCR technique after genomic DNA isolation from maternal blood-EDTA, control group blood-EDTA and spontaneously aborted fetal tissues. Some cases had a single thrombophilic polymorphism, but the rest of the patients and fetal materials had combined thrombophilic polymorphisms. The PAI-1 4G/5G+4G/4G (P= 0.0017), 4G/4G (P= 0.0253), eNOS 894GT+894TT (P=0.0011) genotypes and T allele (P=0.0185), Apo E E3/E4+E3/E2+E2/E4 (P<0.0001) genotypes, E2 (P<0.0001) and E4 (P<0.0001) alleles were higher in spontaneously aborted fetal materials when compared to their mothers and control group. The Factor V Leiden rs6025, Prothrombin G20210A, MTHFR C677T, ACE I/D genotypes were different for each group but not statistically significant due to relatively small size of the samples (P>0.05). Our results indicated that combined thrombophilic gene variations may be associated with increased risk for spontaneous abortions and results need to be confirmed by larger sample size.

Citing Articles

Morphometric and Nanomechanical Features of Platelets from Women with Early Pregnancy Loss Provide New Evidence of the Impact of Inherited Thrombophilia.

Andreeva T, Komsa-Penkova R, Langari A, Krumova S, Golemanov G, Georgieva G Int J Mol Sci. 2021; 22(15).

PMID: 34360543 PMC: 8346153. DOI: 10.3390/ijms22157778.

Maternal methyltetrahydrofolate reductase gene mutation in patients with missed abortions.

Rady H J Taibah Univ Med Sci. 2019; 13(1):93-96.

PMID: 31435309 PMC: 6695003. DOI: 10.1016/j.jtumed.2017.04.004.

Association of Angiotensin-Converting Enzyme Insertion/Deletion Polymorphism with Recurrent Pregnancy Loss: a Meta-Analysis of 26 Case-Control Studies.

Aslbahar F, Neamatzadeh H, Tabatabaiee R, Karimi-Zarchi M, Javaheri A, Mazaheri M Rev Bras Ginecol Obstet. 2018; 40(10):631-641.

PMID: 30352462 PMC: 10316902. DOI: 10.1055/s-0038-1672137.

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