Alteration in Metabolic Signature and Lipid Metabolism in Patients with Angina Pectoris and Myocardial Infarction

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Aug 11

PMID 26258408

Citations 59

Authors

Ju Yeon Park

Sang-Hak Lee

Min-Jeong Shin

Geum-Sook Hwang

Affiliations

Soon will be listed here.

Abstract

Lipid metabolites are indispensable regulators of physiological and pathological processes, including atherosclerosis and coronary artery disease (CAD). However, the complex changes in lipid metabolites and metabolism that occur in patients with these conditions are incompletely understood. We performed lipid profiling to identify alterations in lipid metabolism in patients with angina and myocardial infarction (MI). Global lipid profiling was applied to serum samples from patients with CAD (angina and MI) and age-, sex-, and body mass index-matched healthy subjects using ultra-performance liquid chromatography/quadruple time-of-flight mass spectrometry and multivariate statistical analysis. A multivariate analysis showed a clear separation between the patients with CAD and normal controls. Lysophosphatidylcholine (lysoPC) and lysophosphatidylethanolamine (lysoPE) species containing unsaturated fatty acids and free fatty acids were associated with an increased risk of CAD, whereas species of lysoPC and lyso-alkyl PC containing saturated fatty acids were associated with a decreased risk. Additionally, PC species containing palmitic acid, diacylglycerol, sphingomyelin, and ceramide were associated with an increased risk of MI, whereas PE-plasmalogen and phosphatidylinositol species were associated with a decreased risk. In MI patients, we found strong positive correlation between lipid metabolites related to the sphingolipid pathway, sphingomyelin, and ceramide and acute inflammatory markers (high-sensitivity C-reactive protein). The results of this study demonstrate altered signatures in lipid metabolism in patients with angina or MI. Lipidomic profiling could provide the information to identity the specific lipid metabolites under the presence of disturbed metabolic pathways in patients with CAD.

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References

Mata P, Alonso R, Lopez-Farre A, Ordovas J, Lahoz C, Garces C . Effect of dietary fat saturation on LDL oxidation and monocyte adhesion to human endothelial cells in vitro. Arterioscler Thromb Vasc Biol. 1996; 16(11):1347-55. DOI: 10.1161/01.atv.16.11.1347. View

Glomset J . The mechanism of the plasma cholesterol esterification reaction: plasma fatty acid transferase. Biochim Biophys Acta. 1962; 65:128-35. DOI: 10.1016/0006-3002(62)90156-7. View

Karabina S, Elisaf M, Bairaktari E, Tzallas C, Siamopoulos K, Tselepis A . Increased activity of platelet-activating factor acetylhydrolase in low-density lipoprotein subfractions induces enhanced lysophosphatidylcholine production during oxidation in patients with heterozygous familial hypercholesterolaemia. Eur J Clin Invest. 1997; 27(7):595-602. DOI: 10.1046/j.1365-2362.1997.1570706.x. View

Lozanski G, Berthier F, Kushner I . The sphingomyelin-ceramide pathway participates in cytokine regulation of C-reactive protein and serum amyloid A, but not alpha-fibrinogen. Biochem J. 1998; 328 ( Pt 1):271-5. PMC: 1218917. DOI: 10.1042/bj3280271. View

Clarke C, Snook C, Tani M, Matmati N, Marchesini N, Hannun Y . The extended family of neutral sphingomyelinases. Biochemistry. 2006; 45(38):11247-56. DOI: 10.1021/bi061307z. View

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

Kien C, Bunn J, Poynter M, Stevens R, Bain J, Ikayeva O . A lipidomics analysis of the relationship between dietary fatty acid composition and insulin sensitivity in young adults. Diabetes. 2012; 62(4):1054-63. PMC: 3609566. DOI: 10.2337/db12-0363. View

Pai J, Pischon T, Ma J, Manson J, Hankinson S, Joshipura K . Inflammatory markers and the risk of coronary heart disease in men and women. N Engl J Med. 2004; 351(25):2599-610. DOI: 10.1056/NEJMoa040967. View

Rosenblat M, Vaya J, Shih D, Aviram M . Paraoxonase 1 (PON1) enhances HDL-mediated macrophage cholesterol efflux via the ABCA1 transporter in association with increased HDL binding to the cells: a possible role for lysophosphatidylcholine. Atherosclerosis. 2005; 179(1):69-77. DOI: 10.1016/j.atherosclerosis.2004.10.028. View

10.

Lourida E, Papathanasiou A, Goudevenos J, Tselepis A . The low-density lipoprotein (LDL)-associated PAF-acetylhydrolase activity and the extent of LDL oxidation are important determinants of the autoantibody titers against oxidized LDL in patients with coronary artery disease. Prostaglandins Leukot Essent Fatty Acids. 2006; 75(2):117-26. DOI: 10.1016/j.plefa.2006.03.012. View

11.

Oka H, Kugiyama K, Doi H, Matsumura T, Shibata H, Miles L . Lysophosphatidylcholine induces urokinase-type plasminogen activator and its receptor in human macrophages partly through redox-sensitive pathway. Arterioscler Thromb Vasc Biol. 2000; 20(1):244-50. DOI: 10.1161/01.atv.20.1.244. View

12.

Chisolm 3rd G, Chai Y . Regulation of cell growth by oxidized LDL. Free Radic Biol Med. 2000; 28(12):1697-707. DOI: 10.1016/s0891-5849(00)00227-6. View

13.

Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T . Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. Circulation. 2001; 104(22):2673-8. DOI: 10.1161/hc4601.099485. View

14.

Toborek M, Lee Y, Garrido R, Kaiser S, Hennig B . Unsaturated fatty acids selectively induce an inflammatory environment in human endothelial cells. Am J Clin Nutr. 2002; 75(1):119-25. DOI: 10.1093/ajcn/75.1.119. View

15.

Cui Z, Houweling M . Phosphatidylcholine and cell death. Biochim Biophys Acta. 2003; 1585(2-3):87-96. DOI: 10.1016/s1388-1981(02)00328-1. View

16.

Simons K, Vaz W . Model systems, lipid rafts, and cell membranes. Annu Rev Biophys Biomol Struct. 2004; 33:269-95. DOI: 10.1146/annurev.biophys.32.110601.141803. View

17.

. Nomenclature and criteria for diagnosis of ischemic heart disease. Report of the Joint International Society and Federation of Cardiology/World Health Organization task force on standardization of clinical nomenclature. Circulation. 1979; 59(3):607-9. DOI: 10.1161/01.cir.59.3.607. View

18.

Vas Dias F, Gibney M, Taylor T . The effect of polyunsaturated fatty acids on the n-3 and n-6 series on platelet aggregation and platelet and aortic fatty acid composition in rabbits. Atherosclerosis. 1982; 43(2-3):245-57. DOI: 10.1016/0021-9150(82)90026-0. View

19.

Pelech S, Pritchard P, Brindley D, Vance D . Fatty acids promote translocation of CTP:phosphocholine cytidylyltransferase to the endoplasmic reticulum and stimulate rat hepatic phosphatidylcholine synthesis. J Biol Chem. 1983; 258(11):6782-8. View

20.

Yao Z, Vance D . The active synthesis of phosphatidylcholine is required for very low density lipoprotein secretion from rat hepatocytes. J Biol Chem. 1988; 263(6):2998-3004. View