Hematopoietic Progenitors Are Required for Proper Development of Coronary Vasculature

Overview

Journal J Mol Cell Cardiol

Specialty Cardiology & Vascular Diseases

Date 2015 Aug 5

PMID 26241844

Citations 13

Authors

Gentian Lluri

Vincent Huang

Marlin Touma

Xiaoqian Liu

Andrew W Harmon

Atsushi Nakano

Affiliations

Soon will be listed here.

Abstract

Rationale: During embryogenesis, hematopoietic cells appear in the myocardium prior to the initiation of coronary formation. However, their role is unknown.

Objective: Here we investigate whether pre-existing hematopoietic cells are required for the formation of coronary vasculature.

Methods And Results: As a model of for hematopoietic cell deficient animals, we used Runx1 knockout embryos and Vav1-cre; R26-DTA embryos, latter of which genetically ablates 2/3 of CD45(+) hematopoietic cells. Both Runx1 knockout embryos and Vav1-cre; R26-DTA embryos revealed disorganized, hypoplastic microvasculature of coronary vessels on section and whole-mount stainings. Furthermore, coronary explant experiments showed that the mouse heart explants from Runx1 and Vav1-cre; R26-DTA embryos exhibited impaired coronary formation ex vivo. Interestingly, in both models it appears that epicardial to mesenchymal transition is adversely affected in the absence of hematopoietic progenitors.

Conclusion: Hematopoietic cells are not merely passively transported via coronary vessel, but substantially involved in the induction of the coronary growth. Our findings suggest a novel mechanism of coronary growth.

Citing Articles

Song J, Zhang X, Lv S, Liu M, Hua X, Yue L BMC Cardiovasc Disord. 2023; 23(1):541.

PMID: 37936072 PMC: 10631011. DOI: 10.1186/s12872-023-03583-3.

RUN(X) out of blood: emerging RUNX1 functions beyond hematopoiesis and links to Down syndrome.

Rozen E, Ozeroff C, Allen M Hum Genomics. 2023; 17(1):83.

PMID: 37670378 PMC: 10481493. DOI: 10.1186/s40246-023-00531-2.

Notch and retinoic acid signals regulate macrophage formation from endocardium downstream of Nkx2-5.

Liu N, Kawahira N, Nakashima Y, Nakano H, Iwase A, Uchijima Y Nat Commun. 2023; 14(1):5398.

PMID: 37669937 PMC: 10480477. DOI: 10.1038/s41467-023-41039-6.

Genetics and Molecular Basis of Congenital Heart Defects in Down Syndrome: Role of Extracellular Matrix Regulation.

Mollo N, Scognamiglio R, Conti A, Paladino S, Nitsch L, Izzo A Int J Mol Sci. 2023; 24(3).

PMID: 36769235 PMC: 9918028. DOI: 10.3390/ijms24032918.

MiR-199a-3p Restrains Foaming and Inflammation by Regulating RUNX1 in Macrophages.

Liu M, Cao Y, Hu Y, Zhang Z, Ji S, Shi L Mol Biotechnol. 2022; 64(10):1130-1142.

PMID: 35435591 DOI: 10.1007/s12033-022-00484-2.

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