Immobilization of Yarrowia Lipolytica Lipase on Macroporous Resin Using Different Methods: Characterization of the Biocatalysts in Hydrolysis Reaction

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Aug 5

PMID 26240816

Citations 4

Authors

Jingjing Sun

Yiling Chen

Jun Sheng

Mi Sun

Affiliations

Soon will be listed here.

Abstract

To improve the reusability and organic solvent tolerance of microbial lipase and expand the application of lipase (hydrolysis, esterification, and transesterification), we immobilized marine microbial lipase using different methods and determined the properties of immobilized lipases. Considering the activity and cost of immobilized lipase, the concentration of lipase was fixed at 2 mg/mL. The optimal temperature of immobilized lipases was 40°C and 5°C higher than free lipase. The activities of immobilized lipases were much higher than free lipase at alkaline pH (more than 50% at pH 12). The free lipase lost most activity (35.3%) and immobilized lipases retained more than 46.4% of their initial activity after 3 h heat treatment at 70°C. At alkaline pH, immobilized lipases were more stable than free lipase (more than 60% residue activity at pH 11 for 3 h). Immobilized lipases retained 80% of their activity after 5 cycles and increased enzyme activity (more than 108.7%) after 3 h treatment in tert-butanol. Immobilization of lipase which improved reusability of lipase and provided a chance to expand the application of marine microbial lipase in organic system expanded the application range of lipase to catalyze hydrolysis and esterification in harsh condition.

Citing Articles

Efficient Biocatalytic Synthesis of Chiral Intermediate of Pregabalin Using Immobilized Lipase.

Ding X, Tang X, Zheng R, Zheng Y Biomed Res Int. 2018; 2018:6192059.

PMID: 30515409 PMC: 6236560. DOI: 10.1155/2018/6192059.

SnO hollow nanotubes: a novel and efficient support matrix for enzyme immobilization.

Zahid Anwar M, Kim D, Kumar A, Patel S, Otari S, Mardina P Sci Rep. 2017; 7(1):15333.

PMID: 29127386 PMC: 5681633. DOI: 10.1038/s41598-017-15550-y.

Selection of Lipases for the Synthesis of Biodiesel from Jatropha Oil and the Potential of Microwave Irradiation to Enhance the Reaction Rate.

Souza L, Mendes A, de Castro H Biomed Res Int. 2016; 2016:1404567.

PMID: 27868060 PMC: 5102879. DOI: 10.1155/2016/1404567.

Enzyme Engineering for In Situ Immobilization.

Rehm F, Chen S, Rehm B Molecules. 2016; 21(10).

PMID: 27754434 PMC: 6273058. DOI: 10.3390/molecules21101370.

References

Bernal C, Urrutia P, Illanes A, Wilson L . Hierarchical meso-macroporous silica grafted with glyoxyl groups: opportunities for covalent immobilization of enzymes. N Biotechnol. 2013; 30(5):500-6. DOI: 10.1016/j.nbt.2013.01.011. View

Prlainovic N, Knezevic-Jugovic Z, Mijin D, Bezbradica D . Immobilization of lipase from Candida rugosa on Sepabeads(®): the effect of lipase oxidation by periodates. Bioprocess Biosyst Eng. 2011; 34(7):803-10. DOI: 10.1007/s00449-011-0530-2. View

Manrich A, Galvao C, Jesus C, Giordano R, Giordano R . Immobilization of trypsin on chitosan gels: use of different activation protocols and comparison with other supports. Int J Biol Macromol. 2008; 43(1):54-61. DOI: 10.1016/j.ijbiomac.2007.11.007. View

Dizge N, Aydiner C, Imer D, Bayramoglu M, Tanriseven A, Keskinler B . Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer. Bioresour Technol. 2008; 100(6):1983-91. DOI: 10.1016/j.biortech.2008.10.008. View

Meng X, Xu G, Zhou Q, Wu J, Yang L . Highly efficient solvent-free synthesis of 1,3-diacylglycerols by lipase immobilised on nano-sized magnetite particles. Food Chem. 2013; 143:319-24. DOI: 10.1016/j.foodchem.2013.07.132. View

Pahujani S, Kanwar S, Chauhan G, Gupta R . Glutaraldehyde activation of polymer Nylon-6 for lipase immobilization: enzyme characteristics and stability. Bioresour Technol. 2007; 99(7):2566-70. DOI: 10.1016/j.biortech.2007.04.042. View

Zhu S, Wu Y, Yu Z . Immobilization of Candida rugosa lipase on a pH-sensitive support for enantioselective hydrolysis of ketoprofen ester. J Biotechnol. 2005; 116(4):397-401. DOI: 10.1016/j.jbiotec.2004.12.012. View

Royon D, Daz M, Ellenrieder G, Locatelli S . Enzymatic production of biodiesel from cotton seed oil using t-butanol as a solvent. Bioresour Technol. 2006; 98(3):648-53. DOI: 10.1016/j.biortech.2006.02.021. View

Mateo , Abian , GUISAN . Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment*. Enzyme Microb Technol. 2000; 26(7):509-515. DOI: 10.1016/s0141-0229(99)00188-x. View

10.

Anobom C, Pinheiro A, De-Andrade R, Aguieiras E, Andrade G, Moura M . From structure to catalysis: recent developments in the biotechnological applications of lipases. Biomed Res Int. 2014; 2014:684506. PMC: 3982246. DOI: 10.1155/2014/684506. View

11.

Sharma R, Chisti Y, Banerjee U . Production, purification, characterization, and applications of lipases. Biotechnol Adv. 2003; 19(8):627-62. DOI: 10.1016/s0734-9750(01)00086-6. View

12.

Guzik U, Hupert-Kocurek K, Wojcieszynska D . Immobilization as a strategy for improving enzyme properties-application to oxidoreductases. Molecules. 2014; 19(7):8995-9018. PMC: 6271243. DOI: 10.3390/molecules19078995. View

13.

Severac E, Galy O, Turon F, Pantel C, Condoret J, Monsan P . Selection of CalB immobilization method to be used in continuous oil transesterification: analysis of the economical impact. Enzyme Microb Technol. 2011; 48(1):61-70. DOI: 10.1016/j.enzmictec.2010.09.008. View

14.

Ghattas N, Abidi F, Galai S, Marzouki M, Salah A . Monoolein production by triglycerides hydrolysis using immobilized Rhizopus oryzae lipase. Int J Biol Macromol. 2014; 68:1-6. DOI: 10.1016/j.ijbiomac.2014.04.017. View

15.

Jenab E, Temelli F, Curtis J . Lipase-catalysed interesterification between canola oil and fully hydrogenated canola oil in contact with supercritical carbon dioxide. Food Chem. 2013; 141(3):2220-8. DOI: 10.1016/j.foodchem.2013.04.079. View

16.

Sassolas A, Blum L, Leca-Bouvier B . Immobilization strategies to develop enzymatic biosensors. Biotechnol Adv. 2011; 30(3):489-511. DOI: 10.1016/j.biotechadv.2011.09.003. View

17.

Yan Y, Zhang X, Chen D . Enhanced catalysis of Yarrowia lipolytica lipase LIP2 immobilized on macroporous resin and its application in enrichment of polyunsaturated fatty acids. Bioresour Technol. 2013; 131:179-87. DOI: 10.1016/j.biortech.2012.12.092. View

18.

Munoz Solano D, Hoyos P, Hernaiz M, Alcantara A, Sanchez-Montero J . Industrial biotransformations in the synthesis of building blocks leading to enantiopure drugs. Bioresour Technol. 2012; 115:196-207. DOI: 10.1016/j.biortech.2011.11.131. View

19.

Hernandez K, Garcia-Galan C, Fernandez-Lafuente R . Simple and efficient immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads. Enzyme Microb Technol. 2011; 49(1):72-8. DOI: 10.1016/j.enzmictec.2011.03.002. View

20.

Chen J, Wu W . Regeneration of immobilized Candida antarctica lipase for transesterification. J Biosci Bioeng. 2005; 95(5):466-9. DOI: 10.1016/s1389-1723(03)80046-4. View