MUC-1 Expression in Pleomorphic Adenomas Using Two Human Milk Fat Globule Protein Membrane Antibodies (HMFG-1 and HMFG-2)

Overview

Journal J Appl Oral Sci

Specialty Dentistry

Date 2015 Jul 30

PMID 26221920

Authors

Santa Ponce-Bravo

Constantino Ledesma-Montes

Maricela Garces-Ortiz

Affiliations

Soon will be listed here.

Abstract

Unlabelled: Pleomorphic adenoma (PA) is the most common salivary gland tumor and its microscopic features and histogenesis are a matter of debate. Human milk fat globule protein membrane (HMFG) monoclonal antibodies (MoAbs) comprise a set of antibodies against the mucin 1 (MUC-1) protein detected in several salivary gland tumors.

Objective: The aim of this study was to assess the immunoexpression of the PA neoplastic cells to MUC-1 protein using HMFG-1 and HMFG-2 MoAbs, contrasting these results with those from normal salivary gland tissue.

Material And Methods: Immunohistochemical detection of MUC-1 protein using HMFG-1 and HMFG-2 MoAbs was made in 5 mm thick, paraffin embedded slides, and the avidin-biotin method was used.

Results: Positivity to HMFG-1 and HMFG-2 MoAbs was found in ductal, squamous metaplastic and neoplastic myoepithelial cells, keratin pearls and intraductal mucous material. Two kinds of myoepithelial cells were identified: classic myoepithelial cells around ducts were negative to both MoAbs, and modified myoepithelial cells were positive to both MoAbs. This last cellular group of the analyzed tumors showed similar MUC-1 immunoexpression to ductal epithelial cells using both HMFG antibodies. Intraductal mucous secretion was also HMFG-1 and HMFG-2 positive.

Conclusions: Our results showed there are two kinds of myoepithelial cells in PA. The first cellular group is represented by the different kinds of neoplastic myoepithelial cells and is HMFG-positive. The second one is HMFG-negative and represented by the neoplastic myoepithelial cells located around the ducts.

References

Ocanto R, Acevedo A . Intraoral minor salivary gland tumors: a retrospective study of 62 cases in a Venezuelan population. J Oral Pathol Med. 1996; 25(1):1-4. DOI: 10.1111/j.1600-0714.1996.tb01214.x. View

Simpson R, Jones H, Beasley P . Benign myoepithelioma of the salivary glands: a true entity?. Histopathology. 1995; 27(1):1-9. DOI: 10.1111/j.1365-2559.1995.tb00284.x. View

Johnson J, Lee J, Cotton D, Lee W, Parsons M . Dimorphic immunohistochemical staining in ocular sebaceous neoplasms: a useful diagnostic aid. Eye (Lond). 1999; 13 ( Pt 1):104-8. DOI: 10.1038/eye.1999.19. View

Ponce-Bravo S, Ledesma-Montes C, Morales-Sanchez I . MUC-1 mucin in normal human salivary glands detected by HMFG-1 and HMFG-2 monoclonal antibodies. APMIS. 2008; 116(2):93-8. DOI: 10.1111/j.1600-0463.2008.00771.x. View

Soares A, Demasi A, Altemani A, de Araujo V . Increased mucin 1 expression in recurrence and malignant transformation of salivary gland pleomorphic adenoma. Histopathology. 2011; 58(3):377-82. DOI: 10.1111/j.1365-2559.2011.03758.x. View

Ljung G, Lennernas B, Nordgren H, Holmberg A, Westlin J, Nilsson S . Human milk fat globule antigen 1 (HMFG1) expression in prostatic carcinoma and immunotargeting with a radiolabelled monoclonal anti-HMFG1 antibody. Anticancer Res. 2000; 20(4):2321-4. View

Saga K . Histochemical and immunohistochemical markers for human eccrine and apocrine sweat glands: an aid for histopathologic differentiation of sweat gland tumors. J Investig Dermatol Symp Proc. 2002; 6(1):49-53. DOI: 10.1046/j.0022-202x.2001.00005.x. View

Simpson R, Pereira E, Ribeiro A, Abdulkadir A, Reis-Filho J . Polymorphous low-grade adenocarcinoma of the salivary glands with transformation to high-grade carcinoma. Histopathology. 2002; 41(3):250-9. DOI: 10.1046/j.1365-2559.2002.01439.x. View

Ledesma-Montes C, Garces-Ortiz M . Salivary gland tumours in a Mexican sample. A retrospective study. Med Oral. 2002; 7(5):324-30. View

10.

Yang G, Liao J, Cassai N, Smolka A, Sidhu G . Parietal cell carcinoma of gastric cardia: immunophenotype and ultrastructure. Ultrastruct Pathol. 2003; 27(2):87-94. DOI: 10.1080/01913120309923. View

11.

Hamada T, Matsukita S, Goto M, Kitajima S, Batra S, Irimura T . Mucin expression in pleomorphic adenoma of salivary gland: a potential role for MUC1 as a marker to predict recurrence. J Clin Pathol. 2004; 57(8):813-21. PMC: 1770389. DOI: 10.1136/jcp.2003.014043. View

12.

Ceriani R, Thompson K, Peterson J, Abraham S . Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule. Proc Natl Acad Sci U S A. 1977; 74(2):582-6. PMC: 392335. DOI: 10.1073/pnas.74.2.582. View

13.

Taylor-Papadimitriou J, Peterson J, Arklie J, Burchell J, Ceriani R, Bodmer W . Monoclonal antibodies to epithelium-specific components of the human milk fat globule membrane: production and reaction with cells in culture. Int J Cancer. 1981; 28(1):17-21. DOI: 10.1002/ijc.2910280104. View

14.

Arklie J, Bodmer W, Egan M, Millis R . Differentiation antigens expressed by epithelial cells in the lactating breast are also detectable in breast cancers. Int J Cancer. 1981; 28(1):23-9. DOI: 10.1002/ijc.2910280105. View

15.

Mariani-Costantini R, Colnaghi M, Leoni F, Menard S, Cerasoli S, RILKE F . Immunohistochemical reactivity of a monoclonal antibody prepared against human breast carcinoma. Virchows Arch A Pathol Anat Histopathol. 1984; 402(4):389-404. DOI: 10.1007/BF00734636. View

16.

Mills S, Wolfe 3rd J, Weiss M, Swanson P, Wick M, Fowler Jr J . Small cell undifferentiated carcinoma of the urinary bladder. A light-microscopic, immunocytochemical, and ultrastructural study of 12 cases. Am J Surg Pathol. 1987; 11(8):606-17. DOI: 10.1097/00000478-198708000-00004. View

17.

Wrba F, Reiner A, Holzner J, Reiner G, Spona J . Prognostic significance of immunohistochemical parameters in breast carcinomas. Pathol Res Pract. 1988; 183(3):277-83. DOI: 10.1016/S0344-0338(88)80122-5. View

18.

Parham D, SLIDDERS W, Robertson A . Quantitation of human milk fat globule (HMFG1) expression in breast carcinoma and its association with survival. J Clin Pathol. 1988; 41(8):875-9. PMC: 1141619. DOI: 10.1136/jcp.41.8.875. View

19.

Zotter S, Hageman P, Lossnitzer A, van den Tweel J, Hilkens J, Mooi W . Monoclonal antibodies to epithelial sialomucins recognize epitopes at different cellular sites in adenolymphomas of the parotid gland. Int J Cancer Suppl. 1988; 3:38-44. DOI: 10.1002/ijc.2910410809. View

20.

Simpson R, Sarsfield P, Clarke T, Babajews A . Clear cell carcinoma of minor salivary glands. Histopathology. 1990; 17(5):433-8. DOI: 10.1111/j.1365-2559.1990.tb00764.x. View