The Pkn22 Ser/Thr Kinase in Nostoc PCC 7120: Role of FurA and NtcA Regulators and Transcript Profiling Under Nitrogen Starvation and Oxidative Stress

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2015 Jul 30

PMID 26220092

Citations 4

Authors

Fan Yingping

Sylvain Lemeille

Andres Gonzalez

Veronique Risoul

Yann Denis

Pierre Richaud

Otmane Lamrabet

Maria F Fillat

Cheng-Cai Zhang

Amel Latifi

Affiliations

Soon will be listed here.

Abstract

Background: The filamentous cyanobacterium Nostoc sp. strain PCC 7120 can fix N2 when combined nitrogen is not available. Furthermore, it has to cope with reactive oxygen species generated as byproducts of photosynthesis and respiration. We have previously demonstrated the synthesis of Ser/Thr kinase Pkn22 as an important survival response of Nostoc to oxidative damage. In this study we wished to investigate the possible involvement of this kinase in signalling peroxide stress and nitrogen deprivation.

Results: Quantitative RT-PCR experiments revealed that the pkn22 gene is induced in response to peroxide stress and to combined nitrogen starvation. Electrophoretic motility assays indicated that the pkn22 promoter is recognized by the global transcriptional regulators FurA and NtcA. Transcriptomic analysis comparing a pkn22-insertion mutant and the wild type strain indicated that this kinase regulates genes involved in important cellular functions such as photosynthesis, carbon metabolism and iron acquisition. Since metabolic changes may lead to oxidative stress, we investigated whether this is the case with nitrogen starvation. Our results rather invalidate this hypothesis thereby suggesting that the function of Pkn22 under nitrogen starvation is independent of its role in response to peroxide stress.

Conclusions: Our analyses have permitted a more complete functional description of Ser/Thr kinase in Nostoc. We have decrypted the transcriptional regulation of the pkn22 gene, and analysed the whole set of genes under the control of this kinase in response to the two environmental changes often encountered by cyanobacteria in their natural habitat: oxidative stress and nitrogen deprivation.

Citing Articles

The Pkn22 Kinase of PCC 7120 Is Required for Cell Differentiation via the Phosphorylation of HetR on a Residue Highly Conserved in Genomes of Heterocyst-Forming Cyanobacteria.

Roumezi B, Xu X, Risoul V, Fan Y, Lebrun R, Latifi A Front Microbiol. 2020; 10:3140.

PMID: 32038573 PMC: 6985446. DOI: 10.3389/fmicb.2019.03140.

Impairment of ntcA gene revealed its role in regulating iron homeostasis, ROS production and cellular phenotype under iron deficiency in cyanobacterium Anabaena sp. PCC 7120.

Singh Kaushik M, Srivastava M, Singh A, Mishra A World J Microbiol Biotechnol. 2017; 33(8):158.

PMID: 28730560 DOI: 10.1007/s11274-017-2323-5.

β-Carboxysome bioinformatics: identification and evolution of new bacterial microcompartment protein gene classes and core locus constraints.

Sommer M, Cai F, Melnicki M, Kerfeld C J Exp Bot. 2017; 68(14):3841-3855.

PMID: 28419380 PMC: 5853843. DOI: 10.1093/jxb/erx115.

Multiplicity and specificity of siderophore uptake in the cyanobacterium Anabaena sp. PCC 7120.

Rudolf M, Stevanovic M, Kranzler C, Pernil R, Keren N, Schleiff E Plant Mol Biol. 2016; 92(1-2):57-69.

PMID: 27325117 DOI: 10.1007/s11103-016-0495-2.

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