Quantitative Proteomics Identifies Serum Response Factor Binding Protein 1 As a Host Factor for Hepatitis C Virus Entry

Overview

Journal Cell Rep

Publisher Cell Press

Specialties Biology
Cell Biology
Molecular Biology

Date 2015 Jul 28

PMID 26212323

Citations 33

Authors

Gisa Gerold

Felix Meissner

Janina Bruening

Kathrin Welsch

Paula M Perin

Thomas F Baumert

Florian W Vondran

Lars Kaderali

Joseph Marcotrigiano

Abdul G Khan

Matthias Mann

Charles M Rice

Thomas Pietschmann

Affiliations

Soon will be listed here.

Abstract

Hepatitis C virus (HCV) enters human hepatocytes through a multistep mechanism involving, among other host proteins, the virus receptor CD81. How CD81 governs HCV entry is poorly characterized, and CD81 protein interactions after virus binding remain elusive. We have developed a quantitative proteomics protocol to identify HCV-triggered CD81 interactions and found 26 dynamic binding partners. At least six of these proteins promote HCV infection, as indicated by RNAi. We further characterized serum response factor binding protein 1 (SRFBP1), which is recruited to CD81 during HCV uptake and supports HCV infection in hepatoma cells and primary human hepatocytes. SRFBP1 facilitates host cell penetration by all seven HCV genotypes, but not of vesicular stomatitis virus and human coronavirus. Thus, SRFBP1 is an HCV-specific, pan-genotypic host entry factor. These results demonstrate the use of quantitative proteomics to elucidate pathogen entry and underscore the importance of host protein-protein interactions during HCV invasion.

Citing Articles

Roles of epidermal growth factor receptor, claudin-1 and occludin in multi-step entry of hepatitis C virus into polarized hepatoma spheroids.

So C, Sourisseau M, Sarwar S, Evans M, Randall G PLoS Pathog. 2023; 19(12):e1011887.

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The matrix metalloproteinase ADAM10 supports hepatitis C virus entry and cell-to-cell spread via its sheddase activity.

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Recent Advances in Molecular and Cellular Functions of S100A10.

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Advances in multi-omics research on viral hepatitis.

Xiang Z, Li J, Lu D, Wei X, Xu X Front Microbiol. 2022; 13:987324.

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The Tetraspanin CD81 Is a Host Factor for Chikungunya Virus Replication.

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