Low-Intensity Pulsed Ultrasound Improves the Functional Properties of Cardiac Mesoangioblasts

Overview

Journal Stem Cell Rev Rep

Publisher Springer

Specialty Cell Biology

Date 2015 Jul 24

PMID 26201830

Citations 11

Authors

Aurora Bernal

Laura M Perez

Beatriz De Lucas

Nuria San Martin

Anke Kadow-Romacker

Gustavo Plaza

Kay Raum

Beatriz G Galvez

Affiliations

Soon will be listed here.

Abstract

Cell-based therapy is a promising approach for many diseases, including ischemic heart disease. Cardiac mesoangioblasts are committed vessel-associated progenitors that can restore to a significant, although partial, extent, heart structure and function in a murine model of myocardial infarction. Low-intensity pulsed ultrasound (LIPUS) is a non-invasive form of mechanical energy that can be delivered into biological tissues as acoustic pressure waves, and is widely used for clinical applications including bone fracture healing. We hypothesized that the positive effects of LIPUS on bone and soft tissue, such as increased cell differentiation and cytoskeleton reorganization, could be applied to increase the therapeutic potential of mesoangioblasts for heart repair. In this work, we show that LIPUS stimulation of cardiac mesoangioblasts isolated from mouse and human heart results in significant cellular modifications that provide beneficial effects to the cells, including increased malleability and improved motility. Additionally, LIPUS stimulation increased the number of binucleated cells and induced cardiac differentiation to an extent comparable with 5'-azacytidine treatment. Mechanistically, LIPUS stimulation activated the BMP-Smad signalling pathway and increased the expression of myosin light chain-2 together with upregulation of β1 integrin and RhoA, highlighting a potentially important role for cytoskeleton reorganization. Taken together, these results provide functional evidence that LIPUS might be a useful tool to explore in the field of heart cell therapy.

Citing Articles

Application of low-intensity pulsed ultrasound on tissue resident stem cells: Potential for ophthalmic diseases.

Lin Z, Gao L, Hou N, Zhi X, Zhang Y, Che Z Front Endocrinol (Lausanne). 2023; 14:1153793.

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Biophysical Approaches for Applying and Measuring Biological Forces.

Sun W, Gao X, Lei H, Wang W, Cao Y Adv Sci (Weinh). 2021; 9(5):e2105254.

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Application of low-intensity pulsed therapeutic ultrasound on mesenchymal precursors does not affect their cell properties.

De Lucas B, Perez L, Bernal A, Galvez B PLoS One. 2021; 16(2):e0246261.

PMID: 33571276 PMC: 7877602. DOI: 10.1371/journal.pone.0246261.

Ultrasound Therapy: Experiences and Perspectives for Regenerative Medicine.

De Lucas B, Perez L, Bernal A, Galvez B Genes (Basel). 2020; 11(9).

PMID: 32957737 PMC: 7563547. DOI: 10.3390/genes11091086.

Impact of low-intensity pulsed ultrasound on transcription and metabolite compositions in proliferation and functionalization of human adipose-derived mesenchymal stromal cells.

Huang D, Gao Y, Wang S, Zhang W, Cao H, Zheng L Sci Rep. 2020; 10(1):13690.

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