Pxn-1 and Pxn-2 May Interact Negatively During Neuronal Development and Aging in C. Elegans

Overview

Journal Mol Cells

Publisher Elsevier

Specialties Cell Biology
Molecular Biology

Date 2015 Jul 22

PMID 26194821

Citations 1

Authors

Injeong Cho

Gyu Jin Hwang

Jeong Hoon Cho

Affiliations

Soon will be listed here.

Abstract

C. elegans has two functional peroxidasins (PXN), PXN-1 and PXN-2. PXN-2 is essential to consolidate the extracellular matrix during development and is suggested to interact with PXN-1 antagonistically. pxn-1 is involved in neuronal development and possibly maintenance; therefore, we investigated the relationship between pxn-1 and pxn-2 in neuronal development and in aging. During neuronal development, defects caused by pxn-1 overexpression were suppressed by overexpression of both pxn-1 and pxn-2. In neuronal aging process, pxn-1 mutants showed less age-related neuronal defects, such as neuronal outgrowth, neuronal wavy processes, and enhanced short-term memory performance. In addition, pxn-2 overexpressing animals retained an intact neuronal morphology when compared with age-matched controls. Consistent with these results, overexpression of both pxn-1 and pxn-2 restored the severe neuronal defects present with pxn-1 overexpression. These results implied that there is a negative relationship between pxn-1 and pxn-2 via pxn-1 regulating pxn-2. Therefore, pxn-1 may function in neuronal development and age-related neuronal maintenance through pxn-2.

Citing Articles

Uncoupling Protein, UCP-4 May Be Involved in Neuronal Defects During Aging and Resistance to Pathogens in Caenorhabditis elegans.

Cho I, Hwang G, Cho J Mol Cells. 2016; 39(9):680-6.

PMID: 27646689 PMC: 5050532. DOI: 10.14348/molcells.2016.0125.

References

Tank E, Rodgers K, Kenyon C . Spontaneous age-related neurite branching in Caenorhabditis elegans. J Neurosci. 2011; 31(25):9279-88. PMC: 3148144. DOI: 10.1523/JNEUROSCI.6606-10.2011. View

Pan C, Peng C, Chen C, McIntire S . Genetic analysis of age-dependent defects of the Caenorhabditis elegans touch receptor neurons. Proc Natl Acad Sci U S A. 2011; 108(22):9274-9. PMC: 3107274. DOI: 10.1073/pnas.1011711108. View

Kauffman A, Parsons L, Stein G, Wills A, Kaletsky R, Murphy C . C. elegans positive butanone learning, short-term, and long-term associative memory assays. J Vis Exp. 2011; (49). PMC: 3197297. DOI: 10.3791/2490. View

Peterfi Z, Toth Z, Kovacs H, Lazar E, Sum A, Donko A . Peroxidasin-like protein: a novel peroxidase homologue in the human heart. Cardiovasc Res. 2013; 101(3):393-9. DOI: 10.1093/cvr/cvt256. View

Brenner S . The genetics of Caenorhabditis elegans. Genetics. 1974; 77(1):71-94. PMC: 1213120. DOI: 10.1093/genetics/77.1.71. View

Gotenstein J, Swale R, Fukuda T, Wu Z, Giurumescu C, Goncharov A . The C. elegans peroxidasin PXN-2 is essential for embryonic morphogenesis and inhibits adult axon regeneration. Development. 2010; 137(21):3603-13. PMC: 2964093. DOI: 10.1242/dev.049189. View

Kauffman A, Ashraf J, Corces-Zimmerman M, Landis J, Murphy C . Insulin signaling and dietary restriction differentially influence the decline of learning and memory with age. PLoS Biol. 2010; 8(5):e1000372. PMC: 2872642. DOI: 10.1371/journal.pbio.1000372. View

Toth M, Melentijevic I, Shah L, Bhatia A, Lu K, Talwar A . Neurite sprouting and synapse deterioration in the aging Caenorhabditis elegans nervous system. J Neurosci. 2012; 32(26):8778-90. PMC: 3427745. DOI: 10.1523/JNEUROSCI.1494-11.2012. View

Lee J, Bandyopadhyay J, Il Lee J, Cho I, Park D, Cho J . A role for peroxidasin PXN-1 in aspects of C. elegans development. Mol Cells. 2014; 38(1):51-7. PMC: 4314122. DOI: 10.14348/molcells.2015.2202. View

10.

Chen C, Chen Y, Jiang H, Chen C, Pan C . Neuronal aging: learning from C. elegans. J Mol Signal. 2013; 8(1):14. PMC: 3895751. DOI: 10.1186/1750-2187-8-14. View

11.

Busch S, Silver J . The role of extracellular matrix in CNS regeneration. Curr Opin Neurobiol. 2007; 17(1):120-7. DOI: 10.1016/j.conb.2006.09.004. View

12.

Kamath R, Martinez-Campos M, Zipperlen P, Fraser A, Ahringer J . Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2001; 2(1):RESEARCH0002. PMC: 17598. DOI: 10.1186/gb-2000-2-1-research0002. View

13.

Mello C, Fire A . DNA transformation. Methods Cell Biol. 1995; 48:451-82. View

14.

Soudi M, Zamocky M, Jakopitsch C, Furtmuller P, Obinger C . Molecular evolution, structure, and function of peroxidasins. Chem Biodivers. 2012; 9(9):1776-93. PMC: 3533774. DOI: 10.1002/cbdv.201100438. View

15.

Fidler A, Vanacore R, Chetyrkin S, Pedchenko V, Bhave G, Yin V . A unique covalent bond in basement membrane is a primordial innovation for tissue evolution. Proc Natl Acad Sci U S A. 2013; 111(1):331-6. PMC: 3890831. DOI: 10.1073/pnas.1318499111. View

16.

Nelson R, Fessler L, Takagi Y, Blumberg B, Keene D, OLSON P . Peroxidasin: a novel enzyme-matrix protein of Drosophila development. EMBO J. 1994; 13(15):3438-47. PMC: 395246. DOI: 10.1002/j.1460-2075.1994.tb06649.x. View

17.

Jin Y, Jorgensen E, Hartwieg E, Horvitz H . The Caenorhabditis elegans gene unc-25 encodes glutamic acid decarboxylase and is required for synaptic transmission but not synaptic development. J Neurosci. 1999; 19(2):539-48. PMC: 6782196. View

18.

Bargmann C, Hartwieg E, Horvitz H . Odorant-selective genes and neurons mediate olfaction in C. elegans. Cell. 1993; 74(3):515-27. DOI: 10.1016/0092-8674(93)80053-h. View