Rosmarinic Acid and Arbutin Suppress Osteoclast Differentiation by Inhibiting Superoxide and Downregulation in RAW 264.7 Cells

Overview

Journal Biomed Rep

Specialty Biochemistry

Date 2015 Jul 15

PMID 26171153

Citations 12

Authors

Akina Omori

Yoshitaka Yoshimura

Yoshiaki Deyama

Kuniaki Suzuki

Affiliations

Soon will be listed here.

Abstract

The present study investigated the effect of the natural polyphenols, rosmarinic acid and arbutin, on osteoclast differentiation in RAW 264.7 cells. Rosmarinic acid and arbutin suppressed osteoclast differentiation and had no cytotoxic effect on osteoclast precursor cells. Rosmarinic acid and arbutin inhibited superoxide production in a dose-dependent manner. mRNA expression of the master regulator of osteoclastogenesis, nuclear factor of activated T cells cytoplasmic 1 () and the osteoclast marker genes, matrix metalloproteinase-9, tartrate-resistant acid phosphatase and cathepsin-K, decreased following treatments with rosmarinic acid and arbutin. Furthermore, resorption activity decreased with the number of osteoclasts. These results suggest that rosmarinic acid and arbutin may be useful for the prevention and treatment of bone diseases, such as osteoporosis, through mechanisms involving inhibition of superoxide and downregulation of .

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