A Novel, Stable, Estradiol-Stimulating, Osteogenic Yam Protein with Potential for the Treatment of Menopausal Syndrome

Overview

Journal Sci Rep

Specialty Science

Date 2015 Jul 11

PMID 26160710

Citations 11

Authors

Kam Lok Wong

Yau Ming Lai

Ka Wan Li

Kai Fai Lee

Tzi Bun Ng

Ho Pan Cheung

Yan Bo Zhang

Lixing Lao

Ricky Ngok-Shun Wong

Pang Chui Shaw

Jack Ho Wong

Zhang-Jin Zhang

Jenny Ka Wing Lam

Wen-Cai Ye

Y E Wencai

Stephen Cho Wing Sze

Affiliations

Soon will be listed here.

Abstract

A novel protein, designated as DOI, isolated from the Chinese yam (Dioscorea opposita Thunb.) could be the first protein drug for the treatment of menopausal syndrome and an alternative to hormone replacement therapy (HRT), which is known to have undesirable side effects. DOI is an acid- and thermo-stable protein with a distinctive N-terminal sequence Gly-Ile-Gly-Lys-Ile-Thr-Thr-Tyr-Trp-Gly-Gln-Tyr-Ser-Asp-Glu-Pro-Ser-Leu-Thr-Glu. DOI was found to stimulate estradiol biosynthesis in rat ovarian granulosa cells; induce estradiol and progesterone secretion in 16- to 18-month-old female Sprague Dawley rats by upregulating expressions of follicle-stimulating hormone receptor and ovarian aromatase; counteract the progression of osteoporosis and augment bone mineral density; and improve cognitive functioning by upregulating protein expressions of brain-derived neurotrophic factor and TrkB receptors in the prefrontal cortex. Furthermore, DOI did not stimulate the proliferation of breast cancer and ovarian cancer cells, which suggest it could be a more efficacious and safer alternative to HRT.

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