Immunosuppression in Experimental Cryptococcosis in Rats. Induction of Thymic Suppressor Cells

Overview

Journal Mycopathologia

Specialties Microbiology
Pharmacology

Date 1989 Oct 1

PMID 2615801

Citations 3

Authors

C E Sotomayor

H R Rubinstein

L Cervi

C M Riera

D T Masih

Affiliations

Soon will be listed here.

Abstract

The presence of the microorganism, cortical hyperplasia and germinal centers was detected in the thymus of rats infected with 10(7) viable Cryptococcus neoformans cells and immunized at 7 days afterwards with 2.5 mg (0.1 ml) of human serum albumin (HSA) incorporated to complete Freund's adjuvant (CFA) (Group 2). There was no modification of the glandular structure in the thymus of the animals only immunized with HSA-CFA (Group 1). The weight of the thymus of group 2, animals infected and immunized, was increased compared with the weight of the thymus of group 1 animals, this became evident by the increase of the thymic index (TI) (p less than 0.005). This rate was obtained calculating the thymus weight/total body weight ratio x 1000. Thymic cells (10(7) cells in 1 ml) obtained from both groups of animals were transferred to normal syngeneic rats of the same sex. The recipient rats were immunized with HSA-CFA 24 h later and 14 days after the transference, the response of delayed-type hypersensitivity (DTH) was studied in them. It could be observed that the thymic cells coming from group 2 animals were capable of suppressing significatively the afferent pathway of the DTH response to HSA when compared with the response of the animals that received cells coming from group 1 rats (p less than 0.0001).

Citing Articles

Thymus, undernutrition, and infection: Approaching cellular and molecular interactions.

Savino W, Duraes J, Maldonado-Galdeano C, Perdigon G, Mendes-da-Cruz D, Cuervo P Front Nutr. 2022; 9:948488.

PMID: 36225882 PMC: 9549110. DOI: 10.3389/fnut.2022.948488.

Severe Changes in Thymic Microenvironment in a Chronic Experimental Model of Paracoccidioidomycosis.

Alves da Costa T, Di Gangi R, Thome R, Felisbino M, Bonfanti A, Watanabe Ishikawa L PLoS One. 2016; 11(10):e0164745.

PMID: 27736987 PMC: 5063316. DOI: 10.1371/journal.pone.0164745.

Programmed cell death in thymus during experimental paracoccidioidomycosis.

Souto P, Brito V, Gameiro J, da Cruz-Hofling M, Verinaud L Med Microbiol Immunol. 2003; 192(4):225-9.

PMID: 12687355 DOI: 10.1007/s00430-003-0180-3.

References

Richter M, Talor E . Cells involved in the immune response. XXXIV: Suppressor cells in the thymus of the immunized rabbit capable of secreting a factor which can suppress the secretion of antibodies from antibody-forming cells in vitro. Clin Immunol Immunopathol. 1986; 41(3):461-78. DOI: 10.1016/0090-1229(86)90017-6. View

Murphy J, Mosley R . Regulation of cell-mediated immunity in cryptococcosis. III. Characterization of second-order T suppressor cells (Ts2). J Immunol. 1985; 134(1):577-84. View

RUBEN L, Clothier R, Buenafe A, Needham P, James H, Balls M . In vitro thymus suppression of hemagglutinin production in Xenopus laevis: location, drug and temperature sensitivity. Thymus. 1984; 6(3):143-52. View

Kadish J, BASCH R . Hematopoietic thymocyte precursors. III. A population of thymocytes with the capacity to return ("home") to the thymus. Cell Immunol. 1977; 30(1):12-24. DOI: 10.1016/0008-8749(77)90043-0. View

Sotomayor C, Rubinstein H, Riera C, Masih D . Immunosuppression in experimental cryptococcosis in rats. Induction of afferent T suppressor cells to a non-related antigen. J Med Vet Mycol. 1987; 25(2):67-76. DOI: 10.1080/02681218780000111. View

Ferro M, Riera C . Potentiation of autoimmune response in rats infected with Toxoplasma gondii. Am J Reprod Immunol Microbiol. 1987; 14(2):33-7. DOI: 10.1111/j.1600-0897.1987.tb00114.x. View

Naparstek Y, Holoshitz J, Eisenstein S, Reshef T, Rappaport S, CHEMKE J . Effector T lymphocyte line cells migrate to the thymus and persist there. Nature. 1982; 300(5889):262-4. DOI: 10.1038/300262a0. View

Murphy J . Effects of first-order Cryptococcus-specific T-suppressor cells on induction of cells responsible for delayed-type hypersensitivity. Infect Immun. 1985; 48(2):439-45. PMC: 261339. DOI: 10.1128/iai.48.2.439-445.1985. View

Asherson G, Dorf M, Colizzi V, Zembala M, JAMES B . Equivalence of conventional anti-picryl T suppressor factor in the contact sensitivity system and monoclonal anti-NP TsF3: their final non-specific effect via the T acceptor cell. Immunology. 1984; 53(3):491-7. PMC: 1454923. View

10.

Sy M, Miller S, Moorhead J, Claman H . Active suppression of 1-fluoro-2,4-dinitrobenzene-immune T cells. Requirement of an auxiliary T cell induced by antigen. J Exp Med. 1979; 149(5):1197-207. PMC: 2184869. DOI: 10.1084/jem.149.5.1197. View

11.

Sy M, Dietz M, Germain R, Benacerraf B, Greene M . Antigen- and receptor-driven regulatory mechanisms. IV. Idiotype-bearing I-J+ suppressor T cell factors induce second-order suppressor T cells which express anti-idiotypic receptors. J Exp Med. 1980; 151(5):1183-95. PMC: 2185840. DOI: 10.1084/jem.151.5.1183. View

12.

Blau J, Waksman B . IMMUNOLOGICAL RESPONSES FOLLOWING INJECTION OF ANTIGENS IN FREUND'S ADJUVANT INTO THYMUS AND OTHER TISSUES. Immunology. 1964; 7:332-41. PMC: 1423452. View

13.

Sunday M, Benacerraf B, Dorf M . Hapten-specific T cell responses to 4-hydroxy-3-nitrophenyl acetyl. VIII. Suppressor cell pathways in cutaneous sensitivity responses. J Exp Med. 1981; 153(4):811-22. PMC: 2186128. DOI: 10.1084/jem.153.4.811. View

14.

Watson S, Bullock W . Immunoregulation in disseminated histoplasmosis: characterization of the surface phenotype of splenic suppressor T lymphocytes. Infect Immun. 1982; 37(3):940-5. PMC: 347629. DOI: 10.1128/iai.37.3.940-945.1982. View

15.

Masih D, Rubinstein H, Sotomayor C, Ferro M, Riera C . Non-specific immunosuppression in experimental cryptococcosis in rats. Mycopathologia. 1986; 94(2):79-84. DOI: 10.1007/BF00437371. View

16.

Weinberger J, Germain R, Ju S, Greene M, Benacerraf B, Dorf M . Hapten-specific T-cell responses to 4-hydroxy-3-nitrophenyl acetyl. II. Demonstration of idiotypic determinants on suppressor T cells. J Exp Med. 1979; 150(4):761-76. PMC: 2185689. DOI: 10.1084/jem.150.4.761. View

17.

Masih D, Sotomayor C, Rubinstein H, Riera C . Immunosuppression in experimental cryptococcosis in rats. Induction of efferent T suppressor cells to a non-related antigen. Mycopathologia. 1991; 114(3):179-86. DOI: 10.1007/BF00437212. View

18.

Murphy J, Mosley R, Moorhead J . Regulation of cell-mediated immunity in cryptococcosis. II. Characterization of first-order T suppressor cells (Ts1) and induction of second-order suppressor cells. J Immunol. 1983; 130(6):2876-81. View

19.

Mosley R, Murphy J, Cox R . Immunoadsorption of Cryptococcus-specific suppressor T-cell factors. Infect Immun. 1986; 51(3):844-50. PMC: 260975. DOI: 10.1128/iai.51.3.844-850.1986. View

20.

Murphy J, Moorhead J . Regulation of cell-mediated immunity in cryptococcosis. I. Induction of specific afferent T suppressor cells by cryptococcal antigen. J Immunol. 1982; 128(1):276-83. View