Effect of Low-level Laser Therapy on Lung Injury Induced by Hindlimb Ischemia/reperfusion in Rats

Overview

Journal Lasers Med Sci

Publisher Springer

Date 2015 Jul 10

PMID 26155904

Citations 2

Authors

Mohammad Ashrafzadeh Takhtfooladi

Hamed Ashrafzadeh Takhtfooladi

Hamidreza Sedaghatfar

Samaneh Shabani

Affiliations

Soon will be listed here.

Abstract

To investigate the effect of low-level laser therapy (LLLT 650 nm) on the lung remote organ injury induced by hindlimb ischemia/reperfusion (I/R). The experiments were performed on 50 healthy mature male Wistar rats weighing mean 230 ± 20 g. The rats were randomly allocated into five equal groups as follows: normal group (animals nonmanipulated), sham group (operated with no ischemia), laser group (animals nonmanipulated and irradiated with laser), I/R group, and I/R + LLLT group. Rats were prepared for sterile surgery, and then, right hindlimbs were subjected to I/R induced by the femoral artery occlusion for duration of 120 min, followed by a 60-min reperfusion. The LLLT (K30 handheld probe, AZOR, Technica, Russia, 650 nm, 30 mW, surface area = 1 cm(2), 60 S/cm(2), energy density = 1.8 J/cm(2)) was carried out by irradiating the rats over a unique point on the skin over the right upper bronchus for 5 and 15 min after initiating reperfusion for 3 min. At the end of the trial, rats were euthanized under deep anesthesia and the right lung tissues were removed. Myeloperoxidase (MPO) and superoxide dismutase (SOD) activities and nitric oxide (NO), malondialdehyde (MDA), and glutathione (GSH) levels were measured in the lung tissues. The tissue samples were further examined histopathologically under light microscopy. It was found that I/R elevated MPO activity, MDA, and NO levels accompanied by a reduction in SOD activities and GSH levels (P < 0.05). LLLT restored MDA and NO levels, MPO and SOD activity, GSH levels, and lung injury scores (P < 0.05). In light of these findings, the LLLT has alleviated the lung tissue injuries after skeletal muscle I/R in this experimental model.

Citing Articles

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Evaluation of low-level laser therapy on skeletal muscle ischemia-reperfusion in streptozotocin-induced diabetic rats by assaying biochemical markers and histological changes.

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References

Koksel O, Yildirim C, Cinel L, Tamer L, Ozdulger A, Basturk M . Inhibition of poly(ADP-ribose) polymerase attenuates lung tissue damage after hind limb ischemia-reperfusion in rats. Pharmacol Res. 2005; 51(5):453-62. DOI: 10.1016/j.phrs.2004.11.007. View

Granger D . Role of xanthine oxidase and granulocytes in ischemia-reperfusion injury. Am J Physiol. 1988; 255(6 Pt 2):H1269-75. DOI: 10.1152/ajpheart.1988.255.6.H1269. View

Blaisdell F . The pathophysiology of skeletal muscle ischemia and the reperfusion syndrome: a review. Cardiovasc Surg. 2002; 10(6):620-30. DOI: 10.1016/s0967-2109(02)00070-4. View

Takhtfooladi H, Takhtfooladi M, Moayer F, Mobarakeh S . Melatonin attenuates lung injury in a hind limb ischemia-reperfusion rat model. Rev Port Pneumol (2006). 2015; 21(1):30-5. DOI: 10.1016/j.rppnen.2014.01.010. View

Takhtfooladi M, Takhtfooladi H, Khansari M . The effects of low-intensity laser therapy on hepatic ischemia-reperfusion injury in a rat model. Lasers Med Sci. 2014; 29(6):1887-93. DOI: 10.1007/s10103-014-1603-7. View

Takhtfooladi M, Jahanshahi A, Sotoudeh A, Jahanshahi G, Takhtfooladi H, Aslani K . Effect of tramadol on lung injury induced by skeletal muscle ischemia-reperfusion: an experimental study. J Bras Pneumol. 2013; 39(4):434-9. PMC: 4075874. DOI: 10.1590/S1806-37132013000400006. View

Granger D, Korthuis R . Physiologic mechanisms of postischemic tissue injury. Annu Rev Physiol. 1995; 57:311-32. DOI: 10.1146/annurev.ph.57.030195.001523. View

de Lima F, Albertini R, Dantas Y, Maia-Filho A, de Loura Santana C, Castro-Faria-Neto H . Low-level laser therapy restores the oxidative stress balance in acute lung injury induced by gut ischemia and reperfusion. Photochem Photobiol. 2012; 89(1):179-88. DOI: 10.1111/j.1751-1097.2012.01214.x. View

Cortas N, Wakid N . Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem. 1990; 36(8 Pt 1):1440-3. View

10.

Xavier M, David D, de Souza R, Arrieiro A, Miranda H, Santana E . Anti-inflammatory effects of low-level light emitting diode therapy on Achilles tendinitis in rats. Lasers Surg Med. 2010; 42(6):553-8. DOI: 10.1002/lsm.20896. View

11.

de Lima F, Vitoretti L, Coelho F, Albertini R, Breithaupt-Faloppa A, Tavares de Lima W . Suppressive effect of low-level laser therapy on tracheal hyperresponsiveness and lung inflammation in rat subjected to intestinal ischemia and reperfusion. Lasers Med Sci. 2012; 28(2):551-64. DOI: 10.1007/s10103-012-1088-1. View

12.

Sotoudeh A, Takhtfooladi M, Jahanshahi A, Asl A, Takhtfooladi H, Khansari M . Effect of N-acetylcysteine on lung injury induced by skeletal muscle ischemia-reperfusion. Histopathlogical study in rat model. Acta Cir Bras. 2012; 27(2):168-71. DOI: 10.1590/s0102-86502012000200012. View

13.

Abergel R, Lyons R, Castel J, Dwyer R, Uitto J . Biostimulation of wound healing by lasers: experimental approaches in animal models and in fibroblast cultures. J Dermatol Surg Oncol. 1987; 13(2):127-33. DOI: 10.1111/j.1524-4725.1987.tb00510.x. View

14.

Landyshev I, AVDEEVA N, Goborov N, Krasavina N, Tikhonova G, Tkacheva S . [Efficacy of low intensity laser irradiation and sodium nedocromil in the complex treatment of patients with bronchial asthma]. Ter Arkh. 2002; 74(3):25-8. View

15.

Seekamp A, Ward P . Ischemia-reperfusion injury. Agents Actions Suppl. 1993; 41:137-52. View

16.

Takhtfooladi M, Jahanbakhsh F, Takhtfooladi H, Yousefi K, Allahverdi A . Effect of low-level laser therapy (685 nm, 3 J/cm(2)) on functional recovery of the sciatic nerve in rats following crushing lesion. Lasers Med Sci. 2015; 30(3):1047-52. DOI: 10.1007/s10103-015-1709-6. View

17.

Klausner J, Anner H, Paterson I, Kobzik L, Valeri C, Shepro D . Lower torso ischemia-induced lung injury is leukocyte dependent. Ann Surg. 1988; 208(6):761-7. PMC: 1493834. DOI: 10.1097/00000658-198812000-00015. View

18.

Wei H, Frenkel K . Relationship of oxidative events and DNA oxidation in SENCAR mice to in vivo promoting activity of phorbol ester-type tumor promoters. Carcinogenesis. 1993; 14(6):1195-201. DOI: 10.1093/carcin/14.6.1195. View

19.

Bulkley G . Free radical-mediated reperfusion injury: a selective review. Br J Cancer Suppl. 1987; 8:66-73. PMC: 2149484. View

20.

McCord J . Oxygen-derived free radicals in postischemic tissue injury. N Engl J Med. 1985; 312(3):159-63. DOI: 10.1056/NEJM198501173120305. View