Elastase and Metalloproteinase-9 Concentrations in Saliva in Patients with Chronic Periodontitis

Overview

Journal Cent Eur J Immunol

Publisher Termedia

Specialty Allergy & Immunology

Date 2015 Jul 9

PMID 26155148

Citations 6

Authors

Malgorzata Nedzi-Gora

Jolanta Kostrzewa-Janicka

Renata Gorska

Affiliations

Soon will be listed here.

Abstract

Elastase and metalloproteinase-9 (MMP-9) are two of numerous proteolytic enzymes released by neutrophilic granulocytes in the course of periodontitis. The aim of the study was to determine the concentrations of elastase and MMP-9 in saliva in patients with chronic periodontitis compared to healthy individuals. The enzyme-linked immunosorbent assay method was employed to determine the concentrations of elastase and MMP-9 in saliva in patients with chronic periodontitis and with pocket depth (PD) ≥ 6 mm and PD < 6 mm, as well as in saliva of healthy individuals. Significantly higher concentrations of elastase and MMP-9 were observed in patients with periodontitis compared to healthy individuals (p < 0.01). Also a significant difference in elastase concentration in saliva was observed between the PD ≥ 4 mm and PD < 6 mm groups and between the PD ≥ 6 mm and control groups, and statistically significant differences in MMP-9 concentrations between the PD ≥ 6 mm and control groups. No statistically significant differences were observed between the PD < 6 mm and control groups for elastase concentrations in saliva as well as between the PD ≥ 6 mm and PD < 6 mm groups, and also between the PD < 6 mm and control groups for MMP-9 concentrations in saliva. Elastase and MMP-9 concentrations in saliva can be considered as biochemical indicators of severity of periodontitis.

Citing Articles

aMMP-8 POCT vs. Other Potential Biomarkers in Chair-Side Diagnostics and Treatment Monitoring of Severe Periodontitis.

Aji N, Raisanen I, Rathnayake N, Lundy F, Mc Crudden M, Goyal L Int J Mol Sci. 2024; 25(17).

PMID: 39273368 PMC: 11395035. DOI: 10.3390/ijms25179421.

Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis.

Mohammed H, Abdulkareem A, Zardawi F, Gul S Diagnostics (Basel). 2022; 12(10).

PMID: 36292174 PMC: 9600931. DOI: 10.3390/diagnostics12102485.

Salivary MMP-9 Levels in Chronic Periodontitis Patients with Type-II Diabetes Mellitus.

Arshad R, Ismail W, Zara B, Naseer R, Minhas S, Ansari M Molecules. 2022; 27(7).

PMID: 35408573 PMC: 9000607. DOI: 10.3390/molecules27072174.

Comparing periodontitis biomarkers in saliva, oral rinse and gingival crevicular fluid: A pilot study.

Katsiki P, Nazmi K, Loos B, Laine M, Schaap K, Hepdenizli E J Clin Periodontol. 2021; 48(9):1250-1259.

PMID: 33998029 PMC: 8453974. DOI: 10.1111/jcpe.13479.

Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva.

Ahmad Z, Harvey R, Paton J, Standish A, Morona R PLoS One. 2018; 13(1):e0190402.

PMID: 29293606 PMC: 5749783. DOI: 10.1371/journal.pone.0190402.

References

Zafiropoulos G, Flores-de-Jacoby L, Todt G, Kolb G, Havemann K, Tatakis D . Gingival crevicular fluid elastase-inhibitor complex: correlation with clinical indices and subgingival flora. J Periodontal Res. 1991; 26(1):24-32. DOI: 10.1111/j.1600-0765.1991.tb01622.x. View

Gustafsson A, Asman B, Bergstrom K, Soder P . Granulocyte elastase in gingival crevicular fluid. A possible discriminator between gingivitis and periodontitis. J Clin Periodontol. 1992; 19(8):535-40. DOI: 10.1111/j.1600-051x.1992.tb00680.x. View

Dennison D, Van Dyke T . The acute inflammatory response and the role of phagocytic cells in periodontal health and disease. Periodontol 2000. 1997; 14:54-78. DOI: 10.1111/j.1600-0757.1997.tb00192.x. View

Rai B, Kharb S, Jain R, Anand S . Biomarkers of periodontitis in oral fluids. J Oral Sci. 2008; 50(1):53-6. DOI: 10.2334/josnusd.50.53. View

Renvert S, Wikstrom M, Mugrabi M, Kelly A, Claffey N . Association of crevicular fluid elastase-like activity with histologically-confirmed attachment loss in ligature-induced periodontitis in beagle dogs. J Clin Periodontol. 1998; 25(5):368-74. DOI: 10.1111/j.1600-051x.1998.tb02457.x. View

Seguier S, Gogly B, Bodineau A, Godeau G, Brousse N . Is collagen breakdown during periodontitis linked to inflammatory cells and expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human gingival tissue?. J Periodontol. 2001; 72(10):1398-406. DOI: 10.1902/jop.2001.72.10.1398. View

Dahan M, Nawrocki B, Elkaim R, Soell M, Bolcato-Bellemin A, Birembaut P . Expression of matrix metalloproteinases in healthy and diseased human gingiva. J Clin Periodontol. 2001; 28(2):128-36. DOI: 10.1034/j.1600-051x.2001.028002128.x. View

Jin L, Soder P, Asman B, Bergstrom K . Granulocyte elastase in gingival crevicular fluid: improved monitoring of the site-specific response to treatment in patients with destructive periodontitis. J Clin Periodontol. 1995; 22(3):240-6. DOI: 10.1111/j.1600-051x.1995.tb00141.x. View

Gursoy U, Kononen E, Huumonen S, Tervahartiala T, Pussinen P, Suominen A . Salivary type I collagen degradation end-products and related matrix metalloproteinases in periodontitis. J Clin Periodontol. 2012; 40(1):18-25. DOI: 10.1111/jcpe.12020. View

10.

Birkedal-Hansen H, Moore W, Bodden M, Windsor L, DeCarlo A, Engler J . Matrix metalloproteinases: a review. Crit Rev Oral Biol Med. 1993; 4(2):197-250. DOI: 10.1177/10454411930040020401. View

11.

Giannopoulou C, Andersen E, Demeurisse C, CIMASONI G . Neutrophil elastase and its inhibitors in human gingival crevicular fluid during experimental gingivitis. J Dent Res. 1992; 71(2):359-63. DOI: 10.1177/00220345920710020301. View

12.

Ujiie Y, Shimada A, Komatsu K, Gomi K, Oida S, Arai T . Degradation of noncollagenous components by neutrophil elastase reduces the mechanical strength of rat periodontal ligament. J Periodontal Res. 2008; 43(1):22-31. DOI: 10.1111/j.1600-0765.2007.00990.x. View

13.

Eley B, Cox S . A 2-year longitudinal study of elastase in human gingival crevicular fluid and periodontal attachment loss. J Clin Periodontol. 1996; 23(7):681-92. DOI: 10.1111/j.1600-051x.1996.tb00594.x. View

14.

Page R . The role of inflammatory mediators in the pathogenesis of periodontal disease. J Periodontal Res. 1991; 26(3 Pt 2):230-42. DOI: 10.1111/j.1600-0765.1991.tb01649.x. View

15.

Palcanis K, Larjava I, Wells B, Suggs K, Landis J, Chadwick D . Elastase as an indicator of periodontal disease progression. J Periodontol. 1992; 63(4):237-42. DOI: 10.1902/jop.1992.63.4.237. View

16.

Nieminen A, Asikainen S, Torkko H, Kari K, Uitto V, SAXEN L . Value of some laboratory and clinical measurements in the treatment plan for advanced periodontitis. J Clin Periodontol. 1996; 23(6):572-81. DOI: 10.1111/j.1600-051x.1996.tb01827.x. View

17.

Ding Y, Haapasalo M, Kerosuo E, Lounatmaa K, Kotiranta A, Sorsa T . Release and activation of human neutrophil matrix metallo- and serine proteinases during phagocytosis of Fusobacterium nucleatum, Porphyromonas gingivalis and Treponema denticola. J Clin Periodontol. 1997; 24(4):237-48. DOI: 10.1111/j.1600-051x.1997.tb01837.x. View

18.

Jin L, Leung W, Corbet E, Soder B . Relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis. J Clin Periodontol. 2002; 29(7):604-14. DOI: 10.1034/j.1600-051x.2002.290704.x. View

19.

Marcaccini A, Meschiari C, Zuardi L, Sampaio de Sousa T, Taba Jr M, Teofilo J . Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy. J Clin Periodontol. 2009; 37(2):180-90. DOI: 10.1111/j.1600-051X.2009.01512.x. View

20.

Ximenez-Fyvie L, Haffajee A, Socransky S . Microbial composition of supra- and subgingival plaque in subjects with adult periodontitis. J Clin Periodontol. 2000; 27(10):722-32. DOI: 10.1034/j.1600-051x.2000.027010722.x. View