Indole-3-acetic Acid (IAA) Production Trait, a Useful Screening to Select Endophytic and Rhizosphere Competent Bacteria for Rice Growth Promoting Agents

Overview

Journal MethodsX

Specialty Pathology

Date 2015 Jul 8

PMID 26150974

Citations 56

Authors

Hassan Etesami

Hossein Ali Alikhani

Hossein Mirseyed Hosseini

Affiliations

Soon will be listed here.

Abstract

Plants select plant growth promoting rhizobacteria (PGPR) that are competitively fit to occupy compatible niches without causing pathological stress on them. However, when screening bacteria for plant growth promoting (PGP) agents, it is better to select bacteria for achieving the most promising isolates having suitable colonization and PGP traits. In most researches, it has been seen that following incubation, bacterial flora are taken at random from petri dishes for further study. However, this type of selection may remove some superior bacteria in terms of PGP traits and high colonization ability. Therefore, it is essential to study all the isolated bacteria in an economic way and select the best bacteria in terms of PGP traits and high colonization rate. A simple screening method to detect endophytic and rhizosphere bacteria, isolated from the plants in rotation with rice, for rice PGP agents based on a root colonization bioassay and a PGP trait is characterized. •Selected bacterial isolates based on their IAA producing trait have the potential for more PGP and colonization of rice plant.•IAA may be the first PGP trait for screening bacteria isolated from plant rotated with rice for rice PGP agents.•The screening procedure appears to be very effective and less time consuming.

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References

Lugtenberg B, Dekkers L, Bloemberg G . Molecular determinants of rhizosphere colonization by Pseudomonas. Annu Rev Phytopathol. 2001; 39:461-90. DOI: 10.1146/annurev.phyto.39.1.461. View

Patten C, Glick B . Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environ Microbiol. 2002; 68(8):3795-801. PMC: 124051. DOI: 10.1128/AEM.68.8.3795-3801.2002. View

Schwyn B, NEILANDS J . Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987; 160(1):47-56. DOI: 10.1016/0003-2697(87)90612-9. View

Mendes R, Pizzirani-Kleiner A, Araujo W, Raaijmakers J . Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemical characterization of Burkholderia cepacia complex isolates. Appl Environ Microbiol. 2007; 73(22):7259-67. PMC: 2168197. DOI: 10.1128/AEM.01222-07. View

Etesami H, Mirseyed Hosseini H, Alikhani H . Bacterial biosynthesis of 1-aminocyclopropane-1-caboxylate (ACC) deaminase, a useful trait to elongation and endophytic colonization of the roots of rice under constant flooded conditions. Physiol Mol Biol Plants. 2014; 20(4):425-34. PMC: 4185049. DOI: 10.1007/s12298-014-0251-5. View

Chaintreuil C, Giraud E, Prin Y, Lorquin J, Ba A, Gillis M . Photosynthetic bradyrhizobia are natural endophytes of the African wild rice Oryza breviligulata. Appl Environ Microbiol. 2000; 66(12):5437-47. PMC: 92479. DOI: 10.1128/AEM.66.12.5437-5447.2000. View

Penrose D, Glick B . Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant. 2003; 118(1):10-15. DOI: 10.1034/j.1399-3054.2003.00086.x. View

Glickmann E, Dessaux Y . A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria. Appl Environ Microbiol. 1995; 61(2):793-6. PMC: 1388360. DOI: 10.1128/aem.61.2.793-796.1995. View

Chi F, Shen S, Cheng H, Jing Y, Yanni Y, Dazzo F . Ascending migration of endophytic rhizobia, from roots to leaves, inside rice plants and assessment of benefits to rice growth physiology. Appl Environ Microbiol. 2005; 71(11):7271-8. PMC: 1287620. DOI: 10.1128/AEM.71.11.7271-7278.2005. View

10.

Soto M, Sanjuan J, Olivares J . Rhizobia and plant-pathogenic bacteria: common infection weapons. Microbiology (Reading). 2006; 152(Pt 11):3167-3174. DOI: 10.1099/mic.0.29112-0. View

11.

Spaepen S, Vanderleyden J, Remans R . Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS Microbiol Rev. 2007; 31(4):425-48. DOI: 10.1111/j.1574-6976.2007.00072.x. View

12.

Lugtenberg B, Dekkers L . What makes Pseudomonas bacteria rhizosphere competent?. Environ Microbiol. 2001; 1(1):9-13. DOI: 10.1046/j.1462-2920.1999.00005.x. View

13.

Brandl , Lindow . Contribution of indole-3-acetic acid production to the epiphytic fitness of erwinia herbicola . Appl Environ Microbiol. 1998; 64(9):3256-63. PMC: 106718. DOI: 10.1128/AEM.64.9.3256-3263.1998. View

14.

Sevilla M, BURRIS R, Gunapala N, Kennedy C . Comparison of benefit to sugarcane plant growth and 15N2 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif- mutants strains. Mol Plant Microbe Interact. 2001; 14(3):358-66. DOI: 10.1094/MPMI.2001.14.3.358. View

15.

James E, Gyaneshwar P, Mathan N, Barraquio W, Reddy P, Iannetta P . Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67. Mol Plant Microbe Interact. 2002; 15(9):894-906. DOI: 10.1094/MPMI.2002.15.9.894. View

16.

Glick B . Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol Res. 2013; 169(1):30-9. DOI: 10.1016/j.micres.2013.09.009. View

17.

Elvira-Recuenco M, van Vuurde J . Natural incidence of endophytic bacteria in pea cultivars under field conditions. Can J Microbiol. 2000; 46(11):1036-41. DOI: 10.1139/w00-098. View