UV Induced Ubiquitination of the Yeast Rad4-Rad23 Complex Promotes Survival by Regulating Cellular DNTP Pools

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2015 Jul 8

PMID 26150418

Citations 7

Authors

Zheng Zhou

Neil Humphryes

Patrick van Eijk

Raymond Waters

Shirong Yu

Rolf Kraehenbuehl

Edgar Hartsuiker

Simon H Reed

Affiliations

Soon will be listed here.

Abstract

Regulating gene expression programmes is a central facet of the DNA damage response. The Dun1 kinase protein controls expression of many DNA damage induced genes, including the ribonucleotide reductase genes, which regulate cellular dNTP pools. Using a combination of gene expression profiling and chromatin immunoprecipitation, we demonstrate that in the absence of DNA damage the yeast Rad4-Rad23 nucleotide excision repair complex binds to the promoters of certain DNA damage response genes including DUN1, inhibiting their expression. UV radiation promotes the loss of occupancy of the Rad4-Rad23 complex from the regulatory regions of these genes, enabling their induction and thereby controlling the production of dNTPs. We demonstrate that this regulatory mechanism, which is dependent on the ubiquitination of Rad4 by the GG-NER E3 ligase, promotes UV survival in yeast cells. These results support an unanticipated regulatory mechanism that integrates ubiquitination of NER DNA repair factors with the regulation of the transcriptional response controlling dNTP production and cellular survival after UV damage.

Citing Articles

Impact of DNA sequences on DNA 'opening' by the Rad4/XPC nucleotide excision repair complex.

Paul D, Mu H, Tavakoli A, Dai Q, Chakraborty S, He C DNA Repair (Amst). 2021; 107:103194.

PMID: 34428697 PMC: 8934541. DOI: 10.1016/j.dnarep.2021.103194.

Comparative Transcriptome Analyses of Derived From SCID Mice and BALB/c Mice: Clues to the Abnormality in Parasite Growth and Development.

Liu R, Cheng W, Ye F, Zhang Y, Zhong Q, Dong H Front Microbiol. 2020; 11:274.

PMID: 32218772 PMC: 7078119. DOI: 10.3389/fmicb.2020.00274.

Nucleotide Excision Repair Protein Rad23 Regulates Cell Virulence Independent of Rad4 in Candida albicans.

Feng J, Yao S, Dong Y, Hu J, Whiteway M, Feng J mSphere. 2020; 5(1).

PMID: 32075883 PMC: 7031613. DOI: 10.1128/mSphere.00062-20.

Hof1 plays a checkpoint-related role in MMS-induced DNA damage response in .

Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M Mol Biol Cell. 2020; 31(5):348-359.

PMID: 31940254 PMC: 7183792. DOI: 10.1091/mbc.E19-06-0316.

Transcriptional responses to DNA damage.

Silva E, Ideker T DNA Repair (Amst). 2019; 79:40-49.

PMID: 31102970 PMC: 6570417. DOI: 10.1016/j.dnarep.2019.05.002.

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