Bacteriological Assessment of the Hospital Environment in Two Referral Hospitals in Yaoundé-Cameroon

Overview

Journal Pan Afr Med J

Date 2015 Jul 4

PMID 26140067

Citations 5

Authors

Kamga Hortense Gonsu

Etienne Guenou

Michel Toukam

Valantine Ngum Ndze

Calixte Didier Mbakop

Dongmo Norbert Tankeu

Francois Xavier Mbopi-Keou

Samuel Takongmo

Affiliations

Soon will be listed here.

Abstract

Introduction: Many studies still show significant numbers of surgical patients contracting nosocomial infections each year globally with high morbidity and mortality. The aim of this study was to identify potential bacteria reservoirs that may be responsible for nosocomial infection in surgical services in the Yaoundé University Teaching Hospital (YUTH) and the Central Hospital Yaoundé (CHY).

Methods: A cross sectional descriptive study was conducted from June to August 2012. Air, water, and surface samples were collected from two surgical services and subjected to standard bacteriological analysis.

Results: A total of 143 surface samples were collected. Bacteria were isolated in all surfaces except from one trolley sample and a surgical cabinet sample. The predominant species in all services was coagulase negative Staphylococcus (CNS). The average number of colonies was 132. 82 CFU/25 cm(2). The bacteria isolated in the air were similar to those isolated from surfaces. From the 16 water samples cultured, an average of 50.93 CFU/100ml bacteria were isolated. The distribution of isolated species showed a predominance of Burkholderia cepacia.

Conclusion: These results showed the importance of the hospital environment as a potential reservoir and source of nosocomial infections amongst surgical patient at YUTH and CHY, thus we suggest that Public health policy makers in Cameroon must define, publish guidelines and recommendations for monitoring environmental microbiota in health facilities.

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References

Jebrak G, Mangiapan G . [Nosocomial pneumonia]. Presse Med. 1996; 25(20):944-50. View

Carling P, Parry M, Von Beheren S . Identifying opportunities to enhance environmental cleaning in 23 acute care hospitals . Infect Control Hosp Epidemiol. 2008; 29(1):1-7. DOI: 10.1086/524329. View

Talon D . The role of the hospital environment in the epidemiology of multi-resistant bacteria. J Hosp Infect. 1999; 43(1):13-7. DOI: 10.1053/jhin.1999.0613. View

Meunier O, Hernandez C, Piroird M, Heilig R, Steinbach D, Freyd A . [Bacteriological sampling of surfaces: importance of the step of enrichment and the choice of the culture media]. Ann Biol Clin (Paris). 2005; 63(5):481-6. View

Oomaki M, Yorioka K, Oie S, Kamiya A . Staphylococcus aureus contamination on the surface of working tables in ward staff centers and its preventive methods. Biol Pharm Bull. 2006; 29(7):1508-10. DOI: 10.1248/bpb.29.1508. View

Rudnick J, Anderson R, Schable B, Miller J, Jarvis W . Gram-negative bacteremia in open-heart-surgery patients traced to probable tap-water contamination of pressure-monitoring equipment. Infect Control Hosp Epidemiol. 1996; 17(5):281-5. DOI: 10.1086/647296. View

Kim K, FEKETY R, Batts D, Brown D, Cudmore M, Silva Jr J . Isolation of Clostridium difficile from the environment and contacts of patients with antibiotic-associated colitis. J Infect Dis. 1981; 143(1):42-50. DOI: 10.1093/infdis/143.1.42. View

Wenzel R . The Lowbury Lecture. The economics of nosocomial infections. J Hosp Infect. 1995; 31(2):79-87. DOI: 10.1016/0195-6701(95)90162-0. View

Horan T, Culver D, Gaynes R, Jarvis W, Edwards J, Reid C . Nosocomial infections in surgical patients in the United States, January 1986-June 1992. National Nosocomial Infections Surveillance (NNIS) System. Infect Control Hosp Epidemiol. 1993; 14(2):73-80. DOI: 10.1086/646686. View

10.

Ponce-de-Leon S . The needs of developing countries and the resources required. J Hosp Infect. 1991; 18 Suppl A:376-81. DOI: 10.1016/0195-6701(91)90044-9. View

11.

Humphrey T . Microbial contamination of hospital showers and shower water: the effect of an automatic drain valve. J Hosp Infect. 1989; 13(1):55-61. DOI: 10.1016/0195-6701(89)90095-9. View

12.

LIDWELL O, Elson R, Lowbury E, Whyte W, Blowers R, Stanley S . Ultraclean air and antibiotics for prevention of postoperative infection. A multicenter study of 8,052 joint replacement operations. Acta Orthop Scand. 1987; 58(1):4-13. DOI: 10.3109/17453678709146334. View

13.

LIDWELL O, Lowbury E, Whyte W, Blowers R, Stanley S, Lowe D . Airborne contamination of wounds in joint replacement operations: the relationship to sepsis rates. J Hosp Infect. 1983; 4(2):111-31. DOI: 10.1016/0195-6701(83)90041-5. View