Cordycepin Induces Apoptosis and Autophagy in Human Neuroblastoma SK-N-SH and BE(2)-M17 Cells

Overview

Journal Oncol Lett

Specialty Oncology

Date 2015 Jul 3

PMID 26137103

Citations 15

Authors

Yifan Li

Rong Li

Shenglang Zhu

Ruyun Zhou

Lei Wang

Jihui Du

Yong Wang

Bei Zhou

Liwen Mai

Affiliations

Soon will be listed here.

Abstract

Cordycepin, also termed 3'-deoxyadenosine, is a derivative of the nucleoside adenosine that represents a potential novel class of anticancer drugs targeting the 3' untranslated region of RNAs. Cordycepin has been reported to induce apoptosis in certain cancer cell lines, but the effects of cordycepin on human neuroblastoma cells have not been studied. In the present study, an MTT assay revealed that cordycepin inhibits the viability of neuroblastoma SK-N-SH and BE(2)-M17 cells in a dose-dependent manner. In addition, cordycepin increases the early-apoptotic cell population of SK-N-SH cells, as determined by fluorescence-activated cell sorting analysis. The induction of apoptosis in neuroblastoma cells by cordycepin was further confirmed by western blotting, which revealed cleavage of caspase-3 and poly(adenosine diphosphate-ribose) polymerase 1 in the SK-N-SH and BE(2)-M17 cells. Cordycepin also induced the formation of a punctate pattern of light-chain 3 (LC3)-associated green fluorescence in the SK-N-SH cells transfected with a pEGFP-LC3 vector. Furthermore, western blotting revealed cleavage of LC3 A/B in cordycepin-treated neuroblastoma SK-N-SH cells. Taken together, the results indicate that cordycepin significantly increases apoptosis and autophagy in neuroblastoma cells, and may therefore be a drug candidate for neuroblastoma therapy, but requires additional evaluation.

Citing Articles

Treatment with autophagic inhibitors enhances oligonol‑induced apoptotic effects in nasopharyngeal carcinoma cells.

Wu Y, Lin C, Chiu W, Hsieh T, Chang S, Chang Y Biomed Rep. 2024; 21(4):143.

PMID: 39161943 PMC: 11332131. DOI: 10.3892/br.2024.1831.

Glyceroglycolipids from the solid culture of strain LY34 isolated from Tibet of China.

Chen B, Lin J, Xu A, Yu D, Phurbu D, Dai H Mycology. 2022; 13(3):185-194.

PMID: 35938083 PMC: 9354630. DOI: 10.1080/21501203.2022.2036841.

A Systematic Review of the Biological Effects of Cordycepin.

Radhi M, Ashraf S, Lawrence S, Tranholm A, Wellham P, Hafeez A Molecules. 2021; 26(19).

PMID: 34641429 PMC: 8510467. DOI: 10.3390/molecules26195886.

The Role of Autophagy in Anti-Cancer and Health Promoting Effects of Cordycepin.

Chen Y, Chen C, Lin W, Tung C, Chen Y, Yang S Molecules. 2021; 26(16).

PMID: 34443541 PMC: 8400201. DOI: 10.3390/molecules26164954.

Novel formulation development from (Berk.) for management of high-altitude maladies.

Rakhee , Mishra J, Yadav R, Meena D, Arora R, Sharma R 3 Biotech. 2021; 11(1):9.

PMID: 33442508 PMC: 7778651. DOI: 10.1007/s13205-020-02536-3.

References

Maris J . Recent advances in neuroblastoma. N Engl J Med. 2010; 362(23):2202-11. PMC: 3306838. DOI: 10.1056/NEJMra0804577. View

Spix C, Pastore G, Sankila R, Stiller C, Steliarova-Foucher E . Neuroblastoma incidence and survival in European children (1978-1997): report from the Automated Childhood Cancer Information System project. Eur J Cancer. 2006; 42(13):2081-91. DOI: 10.1016/j.ejca.2006.05.008. View

Oh J, Baek Y, Kim S, Hwang H, Hwang H, Lee S . Apoptosis of human hepatocarcinoma (HepG2) and neuroblastoma (SKN-SH) cells induced by polysaccharides-peptide complexes produced by submerged mycelial culture of an entomopathogenic fungus Cordyceps sphecocephala. J Microbiol Biotechnol. 2008; 18(3):512-9. View

Tuli H, Sharma A, Sandhu S, Kashyap D . Cordycepin: a bioactive metabolite with therapeutic potential. Life Sci. 2013; 93(23):863-9. DOI: 10.1016/j.lfs.2013.09.030. View

Gurney J, Ross J, Wall D, Bleyer W, Severson R, Robison L . Infant cancer in the U.S.: histology-specific incidence and trends, 1973 to 1992. J Pediatr Hematol Oncol. 1997; 19(5):428-32. DOI: 10.1097/00043426-199709000-00004. View

Boya P, Gonzalez-Polo R, Casares N, Perfettini J, Dessen P, Larochette N . Inhibition of macroautophagy triggers apoptosis. Mol Cell Biol. 2005; 25(3):1025-40. PMC: 543994. DOI: 10.1128/MCB.25.3.1025-1040.2005. View

Brodeur G, Pritchard J, Berthold F, Carlsen N, Castel V, Castelberry R . Revisions of the international criteria for neuroblastoma diagnosis, staging, and response to treatment. J Clin Oncol. 1993; 11(8):1466-77. DOI: 10.1200/JCO.1993.11.8.1466. View

Chen Y, Hao L, Hung C, Chen J, Leu S, Huang B . Apoptotic effect of cisplatin and cordycepin on OC3 human oral cancer cells. Chin J Integr Med. 2013; 20(8):624-32. DOI: 10.1007/s11655-013-1453-3. View

Choi S, Lim M, Kim K, Jeon B, Song W, Woong Kim T . Cordycepin-induced apoptosis and autophagy in breast cancer cells are independent of the estrogen receptor. Toxicol Appl Pharmacol. 2011; 257(2):165-73. DOI: 10.1016/j.taap.2011.08.030. View

10.

Wu Y, Tan H, Shui G, Bauvy C, Huang Q, Wenk M . Dual role of 3-methyladenine in modulation of autophagy via different temporal patterns of inhibition on class I and III phosphoinositide 3-kinase. J Biol Chem. 2010; 285(14):10850-61. PMC: 2856291. DOI: 10.1074/jbc.M109.080796. View

11.

Diede S . Spontaneous regression of metastatic cancer: learning from neuroblastoma. Nat Rev Cancer. 2014; 14(2):71-2. DOI: 10.1038/nrc3656. View

12.

Kabeya Y, Mizushima N, Ueno T, Yamamoto A, Kirisako T, Noda T . LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 2000; 19(21):5720-8. PMC: 305793. DOI: 10.1093/emboj/19.21.5720. View

13.

Siev M, Weinberg R, Penman S . The selective interruption of nucleolar RNA synthesis in HeLa cells by cordycepin. J Cell Biol. 1969; 41(2):510-20. PMC: 2107749. DOI: 10.1083/jcb.41.2.510. View

14.

Hollerer I, Grund K, Hentze M, Kulozik A . mRNA 3'end processing: A tale of the tail reaches the clinic. EMBO Mol Med. 2014; 6(1):16-26. PMC: 3936486. DOI: 10.1002/emmm.201303300. View

15.

Maris J, Hogarty M, Bagatell R, Cohn S . Neuroblastoma. Lancet. 2007; 369(9579):2106-20. DOI: 10.1016/S0140-6736(07)60983-0. View

16.

Brodeur G, Seeger R, Barrett A, Berthold F, Castleberry R, DAngio G . International criteria for diagnosis, staging, and response to treatment in patients with neuroblastoma. J Clin Oncol. 1988; 6(12):1874-81. DOI: 10.1200/JCO.1988.6.12.1874. View

17.

Szemes M, Dallosso A, Melegh Z, Curry T, Li Y, Rivers C . Control of epigenetic states by WT1 via regulation of de novo DNA methyltransferase 3A. Hum Mol Genet. 2012; 22(1):74-83. PMC: 6296327. DOI: 10.1093/hmg/dds403. View

18.

Lee S, Debnath T, Kim S, Lim B . Anti-cancer effect and apoptosis induction of cordycepin through DR3 pathway in the human colonic cancer cell HT-29. Food Chem Toxicol. 2013; 60:439-47. DOI: 10.1016/j.fct.2013.07.068. View

19.

Yang C, Kao Y, Huang K, Wang C, Lin L . Cordyceps militaris and mycelial fermentation induced apoptosis and autophagy of human glioblastoma cells. Cell Death Dis. 2012; 3:e431. PMC: 3542607. DOI: 10.1038/cddis.2012.172. View

20.

DAngio G, Evans A, KOOP C . Special pattern of widespread neuroblastoma with a favourable prognosis. Lancet. 1971; 1(7708):1046-9. DOI: 10.1016/s0140-6736(71)91606-0. View