A Novel Chimeric Anti-PA Neutralizing Antibody for Postexposure Prophylaxis and Treatment of Anthrax

Overview

Journal Sci Rep

Specialty Science

Date 2015 Jul 3

PMID 26134518

Citations 4

Authors

Siping Xiong

Qi Tang

Xudong Liang

Tingting Zhou

Jin Yang

Peng Liu

Ya Chen

Changjun Wang

Zhenqing Feng

Jin Zhu

Affiliations

Soon will be listed here.

Abstract

Anthrax is a highly lethal infectious disease caused by the bacterium Bacillus anthracis, and the associated shock is closely related to the lethal toxin (LeTx) produced by the bacterium. The central role played by the 63 kDa protective antigen (PA63) region of LeTx in the pathophysiology of anthrax makes it an excellent therapeutic target. In the present study, a human/murine chimeric IgG mAb, hmPA6, was developed by inserting murine antibody variable regions into human constant regions using antibody engineering technology. hmPA6 expressed in 293F cells could neutralize LeTx both in vitro and in vivo. At a dose of 0.3 mg/kg, it could protect all tested rats from a lethal dose of LeTx. Even administration of 0.6 mg/kg hmPA6 48 h before LeTx challenge protected all tested rats. The results indicate that hmPA6 is a potential candidate for clinical application in anthrax treatment.

Citing Articles

Structural Basis for Binding of Neutralizing Antibodies to Binary Toxin.

Goldsmith J, Dewar V, Hermand P, Blais N, McLellan J J Bacteriol. 2023; 205(4):e0045622.

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Different mechanisms of two anti-anthrax protective antigen antibodies and function comparison between them.

Xiong S, Zhou T, Zheng F, Liang X, Cao Y, Wang C BMC Infect Dis. 2019; 19(1):940.

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Human monoclonal anti-protective antigen antibody for the low-dose post-exposure prophylaxis and treatment of Anthrax.

Tang Q, Xiong S, Liang X, Kuai X, Wang Y, Wang C BMC Infect Dis. 2018; 18(1):640.

PMID: 30526504 PMC: 6288905. DOI: 10.1186/s12879-018-3542-6.

A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway.

Mao Y, Wang J, Zhang M, Fan W, Tang Q, Xiong S Oncotarget. 2016; 8(7):10954-10965.

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