Proteomic Profiling of Posterior Longitudinal Ligament of Cervical Spine

Overview

Journal Int J Clin Exp Med

Specialty General Medicine

Date 2015 Jul 2

PMID 26131146

Citations 4

Authors

Ying Zhang

Baifeng Liu

Jiang Shao

Jia Song

Jing Zhang

Affiliations

Soon will be listed here.

Abstract

Objective: To identify putative biomarkers for ossification of posterior longitudinal ligament (OPLL).

Material And Methods: Proteomic analysis was performed in 4 ligament samples from OPLL patients and healthy controls. RT-PCR was used to further verify the proteomic analysis results.

Results: A total of 50 differentially expressed spots were detected in 2-D electrophoresis between the two groups. In protein/peptide analysis, 21 proteins or peptides were finally identified. Besides 13 hematic proteins and 2 unknown proteins, 6 other proteins were differentially expressed. Among them, carbonic anhydrase I, NAD(P) dependent steroid dehydrogenase-like, billiverdin reductase B and alpha-1 collagen VI were down-regulated, while osteoglycin and nebulin-related anchoring protein were up-regulated. The results of NAD(P) dependent steroid dehydrogenase-like, alpha-1 collagen VI and nebulin-related anchoring protein were validated by RT-PCR.

Conclusion: These differentially expressed proteins could play a role in the onset and progression of OPLL.

Citing Articles

Biomarker Research Approach to the Pathogenesis of Ossification of the Spinal Ligament: A Review.

Kawaguchi Y Spine Surg Relat Res. 2022; 6(3):224-232.

PMID: 35800628 PMC: 9200417. DOI: 10.22603/ssrr.2021-0229.

Biomarkers of Ossification of the Spinal Ligament.

Kawaguchi Y Global Spine J. 2019; 9(6):650-657.

PMID: 31448200 PMC: 6693072. DOI: 10.1177/2192568218791799.

Molecular Pathophysiology of Ossification of the Posterior Longitudinal Ligament (OPLL).

Nam D, Lee H, Lee C, Hwang S Biomol Ther (Seoul). 2019; 27(4):342-348.

PMID: 31042677 PMC: 6609106. DOI: 10.4062/biomolther.2019.043.

The Pathogenesis of Ossification of the Posterior Longitudinal Ligament.

Yan L, Gao R, Liu Y, He B, Lv S, Hao D Aging Dis. 2017; 8(5):570-582.

PMID: 28966802 PMC: 5614322. DOI: 10.14336/AD.2017.0201.

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