Assessing the Susceptibility Status of Mosquitoes (Diptera: Culicidae) in a Dirofilariasis Focus, Northwestern Iran

Overview

Journal J Arthropod Borne Dis

Publisher Tehran University of Medical Sciences

Date 2015 Jun 27

PMID 26114140

Citations 16

Authors

Abolfazl Ataie

Seyed Hassan Moosa-Kazemi

Hassan Vatandoost

Mohammad Reza Yaghoobi-Ershadi

Hasan Bakhshi

Mehdi Anjomruz

Affiliations

Soon will be listed here.

Abstract

Background: Mosquitoes are considered as the vectors of dirofilariasis and some vector borne disease in Iran. The objective of this study was to determine the susceptibility level of the vectors to various insecticides recommended by WHO for any control measures in an endemic area in northwestern Iran.

Methods: Mosquito larval and adult collections were carried out using different methods provided by WHO including dipping and hand catch techniques. The susceptibility level was assessed to DDT 4%, malathion 5%, propoxur 0.1%, deltamethrin 0.05% and lambda-cyhalothrin 0.05%.

Results: Totally, 749 adults and 5060 larvae of Culicidae mosquitoes were collected comprising seven species of adult and larvae, including: Anopheles claviger, An. maculipennis, An. sacharovi, Culex hortensis, Cx. pipiens, Cx. theileri and Culiseta longiaerolata. Frequency of larvae and adults of An. maculipennis was very low, so susceptibility tests on this species did not performed. Results showed that Cx. theileri, Cs. longiaerolata and Cx. pipiens were resistant to DDT 4%, lambda-cyhalothrin 0.05%, and propoxur 0.1% whereas found tolerant to deltamethrin 0.05% and malathion 5%. The LT50 and LT90 values for five insecticides were calculated.

Conclusion: We suggest the same study in different parts of the world to obtain the data due to bionomic and susceptibility status of dirofilariasis vectors. This information will help the health authorities for monitoring and evaluation of control measures.

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References

Chandre F, Darriet F, Darder M, Cuany A, Doannio J, Pasteur N . Pyrethroid resistance in Culex quinquefasciatus from west Africa. Med Vet Entomol. 1998; 12(4):359-66. DOI: 10.1046/j.1365-2915.1998.00120.x. View

Ben Cheikh H, Marquine M, Pasteur N . Resistance to organophosphorus and pyrethroid insecticides in Culex pipiens (Diptera: Culicidae) from Tunisia. J Med Entomol. 1998; 35(3):251-60. DOI: 10.1093/jmedent/35.3.251. View

Sadighian A . Helminth parasites of stray dogs and jackals in Shahsavar area, caspian region, Iran. J Parasitol. 1969; 55(2):372-4. View

Slocombe J, Villeneuve A . Heartworm in dogs in Canada in 1991. Can Vet J. 1993; 34(10):630-3. PMC: 1686628. View

Barr A, KARTMAN L . Biometrical notes on the hybridization of Culex pipiens L. and C. quinquefasciatus Say. J Parasitol. 1951; 37(4):419-20. View

Genchi C, Rinaldi L, Cascone C, Mortarino M, Cringoli G . Is heartworm disease really spreading in Europe?. Vet Parasitol. 2005; 133(2-3):137-48. DOI: 10.1016/j.vetpar.2005.04.009. View

Bazzocchi C, Ceciliani F, McCall J, Ricci I, Genchi C, Bandi C . Antigenic role of the endosymbionts of filarial nematodes: IgG response against the Wolbachia surface protein in cats infected with Dirofilaria immitis. Proc Biol Sci. 2001; 267(1461):2511-6. PMC: 1690852. DOI: 10.1098/rspb.2000.1313. View

Zaim M . Malaria control in Iran--present and future. J Am Mosq Control Assoc. 1987; 3(3):392-6. View

Lotfi M . Iranian species of genus Culex (Culinae:Diptera). Bull Soc Pathol Exot Filiales. 1970; 63(3):399-403. View

10.

Reifur L, Thomaz-Soccol V, Montiani-Ferreira F . Epidemiological aspects of filariosis in dogs on the coast of Paraná state, Brazil: with emphasis on Dirofilaria immitis. Vet Parasitol. 2004; 122(4):273-86. DOI: 10.1016/j.vetpar.2004.05.017. View

11.

Venco L, McCall J, Guerrero J, Genchi C . Efficacy of long-term monthly administration of ivermectin on the progress of naturally acquired heartworm infections in dogs. Vet Parasitol. 2004; 124(3-4):259-68. DOI: 10.1016/j.vetpar.2004.06.024. View

12.

Rodriguez M, Ortiz E, Bisset J, Hemingway J, Saledo E . Changes in malathion and pyrethroid resistance after cypermethrin selection of Culex quinquefasciatus field populations of Cuba. Med Vet Entomol. 1993; 7(2):117-21. DOI: 10.1111/j.1365-2915.1993.tb00663.x. View

13.

SHAHGUDIAN E . A key to the anophelines of Iran. Acta Med Iran. 1960; 3:38-48. View

14.

Enayati A, Vatandoost H, Ladonni H, Townson H, Hemingway J . Molecular evidence for a kdr-like pyrethroid resistance mechanism in the malaria vector mosquito Anopheles stephensi. Med Vet Entomol. 2003; 17(2):138-44. DOI: 10.1046/j.1365-2915.2003.00418.x. View

15.

Mehrabani D, Oryan A, Sadjjadi S . Prevalence of Echinococcus granulosus infection in stray dogs and herbivores in Shiraz, Iran. Vet Parasitol. 1999; 86(3):217-20. DOI: 10.1016/s0304-4017(99)00151-x. View

16.

Yildirim A, Ica A, Atalay O, Duzlu O, Inci A . Prevalence and epidemiological aspects of Dirofilaria immitis in dogs from Kayseri Province, Turkey. Res Vet Sci. 2006; 82(3):358-63. DOI: 10.1016/j.rvsc.2006.08.006. View

17.

Nikookar S, Moosa-Kazemi S, Oshaghi M, Yaghoobi-Ershadi M, Vatandoost H, Kianinasab A . Species composition and diversity of mosquitoes in neka county, mazandaran province, northern iran. Iran J Arthropod Borne Dis. 2012; 4(2):26-34. PMC: 3385557. View

18.

Aranda C, Panyella O, Eritja R, Castella J . Canine filariasis. Importance and transmission in the Baix Llobregat area, Barcelona (Spain). Vet Parasitol. 1998; 77(4):267-75. DOI: 10.1016/s0304-4017(98)00109-5. View

19.

Peribanez M, Lucientes J, Arce S, Morales M, Castillo J, Gracia M . Histochemical differentiation of Dirofilaria immitis, Dirofilaria repens and Acanthocheilonema dracunculoides microfilariae by staining with a commercial kit, Leucognost-SP. Vet Parasitol. 2001; 102(1-2):173-5. DOI: 10.1016/s0304-4017(01)00516-7. View

20.

Azari-Hamidian S . Checklist of Iranian mosquitoes (Diptera: Culicidae). J Vector Ecol. 2008; 32(2):235-42. DOI: 10.3376/1081-1710(2007)32[235:coimdc]2.0.co;2. View