SNAP23/25 and VAMP2 Mediate Exocytic Event of Transferrin Receptor-containing Recycling Vesicles

Overview

Journal Biol Open

Specialty Biology

Date 2015 Jun 21

PMID 26092867

Citations 15

Authors

Keiji Kubo

Minako Kobayashi

Shohei Nozaki

Chikako Yagi

Kiyotaka Hatsuzawa

Yohei Katoh

Hye-Won Shin

Senye Takahashi

Kazuhisa Nakayama

Affiliations

Soon will be listed here.

Abstract

We recently showed that Rab11 is involved not only in formation of recycling vesicles containing the transferrin (Tfn)-transferrin receptor (TfnR) complex at perinuclear recycling endosomes but also in tethering of recycling vesicles to the plasma membrane (PM) in concert with the exocyst tethering complex. We here aimed at identifying SNARE proteins responsible for fusion of Tfn-TfnR-containing recycling vesicles with the PM, downstream of the exocyst. We showed that exocyst subunits, Sec6 and Sec8, can interact with SNAP23 and SNAP25, both of which are PM-localizing Qbc-SNAREs, and that depletion of SNAP23 and/or SNAP25 in HeLa cells suppresses fusion of Tfn-TfnR-containing vesicles with the PM, leading to accumulation of the vesicles at the cell periphery. We also found that VAMP2, an R-SNARE, is colocalized with endocytosed Tfn on punctate endosomal structures, and that its depletion in HeLa cells suppresses recycling vesicle exocytosis. These observations indicate that fusion of recycling vesicles with the PM downstream of the exocyst is mediated by SNAP23/25 and VAMP2, and provide novel insight into non-neuronal roles of VAMP2 and SNAP25.

Citing Articles

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