Impact of Recent Innovations in the Use of Mass Cytometry in Support of Drug Development

Overview

Journal Drug Discov Today

Specialty Pharmacology

Date 2015 Jun 21

PMID 26092491

Citations 10

Authors

Ala F Nassar

Hideki Ogura

Adam V Wisnewski

Affiliations

Soon will be listed here.

Abstract

Cytometry by time-of-flight (CyTOF) is a novel technology for the real-time analysis of single cells. CyTOF is a significant advance in fields including immunology, hematology, and oncology. It resolves multiple metal-conjugated probes per cell with minimal signal overlap, which maximizes the information obtained from each individual sample. CyTOF provides the ability to phenotypically and functionally profile cells from normal and diseased states. Single cell technologies enable researchers to measure the effects of a drug at the single cell level and better understand its mechanism of action. Here, we discuss novel instruments for the analysis of individual biological cells, the impact of recent innovations in support of drug development, and the important roles of CyTOF in drug profiling.

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References

Becher B, Schlitzer A, Chen J, Mair F, Sumatoh H, Teng K . High-dimensional analysis of the murine myeloid cell system. Nat Immunol. 2014; 15(12):1181-9. DOI: 10.1038/ni.3006. View

Dieterlen M, Eberhardt K, Tarnok A, Bittner H, Barten M . Flow cytometry-based pharmacodynamic monitoring after organ transplantation. Methods Cell Biol. 2011; 103:267-84. DOI: 10.1016/B978-0-12-385493-3.00011-5. View

Rothwell P, Fuccillo M, Maxeiner S, Hayton S, Gokce O, Lim B . Autism-associated neuroligin-3 mutations commonly impair striatal circuits to boost repetitive behaviors. Cell. 2014; 158(1):198-212. PMC: 4120877. DOI: 10.1016/j.cell.2014.04.045. View

Shi S, Leites C, He D, Schwartz D, Moy W, Shi J . MicroRNA-9 and microRNA-326 regulate human dopamine D2 receptor expression, and the microRNA-mediated expression regulation is altered by a genetic variant. J Biol Chem. 2014; 289(19):13434-44. PMC: 4036351. DOI: 10.1074/jbc.M113.535203. View

Chini V . Micro-RNAs and next generation sequencing: new perspectives in heart failure. Clin Chim Acta. 2014; 443:114-9. DOI: 10.1016/j.cca.2014.11.020. View

Bennett R, Ysasi A, Belle J, Wagner W, Konerding M, Blainey P . Laser microdissection of the alveolar duct enables single-cell genomic analysis. Front Oncol. 2014; 4:260. PMC: 4173809. DOI: 10.3389/fonc.2014.00260. View

Fu J, Stone N, Liu L, Spencer C, Qian L, Hayashi Y . Direct reprogramming of human fibroblasts toward a cardiomyocyte-like state. Stem Cell Reports. 2013; 1(3):235-47. PMC: 3849259. DOI: 10.1016/j.stemcr.2013.07.005. View

Melchor L, Brioli A, Wardell C, Murison A, Potter N, Kaiser M . Single-cell genetic analysis reveals the composition of initiating clones and phylogenetic patterns of branching and parallel evolution in myeloma. Leukemia. 2014; 28(8):1705-15. DOI: 10.1038/leu.2014.13. View

Horowitz A, Strauss-Albee D, Leipold M, Kubo J, Nemat-Gorgani N, Dogan O . Genetic and environmental determinants of human NK cell diversity revealed by mass cytometry. Sci Transl Med. 2013; 5(208):208ra145. PMC: 3918221. DOI: 10.1126/scitranslmed.3006702. View

10.

Ornatsky O, Lou X, Nitz M, Schafer S, Sheldrick W, Baranov V . Study of cell antigens and intracellular DNA by identification of element-containing labels and metallointercalators using inductively coupled plasma mass spectrometry. Anal Chem. 2008; 80(7):2539-47. DOI: 10.1021/ac702128m. View

11.

Sun H, Olsen T, Zhu J, Tao J, Ponnaiya B, Amundson S . A Bead-Based Microfluidic Approach to Integrated Single-Cell Gene Expression Analysis by Quantitative RT-PCR. RSC Adv. 2015; 5(7):4886-4893. PMC: 4394375. DOI: 10.1039/C4RA13356K. View

12.

Irish J, Hovland R, Krutzik P, Perez O, Bruserud O, Gjertsen B . Single cell profiling of potentiated phospho-protein networks in cancer cells. Cell. 2004; 118(2):217-28. DOI: 10.1016/j.cell.2004.06.028. View

13.

Bandura D, Baranov V, Ornatsky O, Antonov A, Kinach R, Lou X . Mass cytometry: technique for real time single cell multitarget immunoassay based on inductively coupled plasma time-of-flight mass spectrometry. Anal Chem. 2009; 81(16):6813-22. DOI: 10.1021/ac901049w. View

14.

Swadling L, Capone S, Antrobus R, Brown A, Richardson R, Newell E . A human vaccine strategy based on chimpanzee adenoviral and MVA vectors that primes, boosts, and sustains functional HCV-specific T cell memory. Sci Transl Med. 2014; 6(261):261ra153. PMC: 4669853. DOI: 10.1126/scitranslmed.3009185. View

15.

Gaudilliere B, Fragiadakis G, Bruggner R, Nicolau M, Finck R, Tingle M . Clinical recovery from surgery correlates with single-cell immune signatures. Sci Transl Med. 2014; 6(255):255ra131. PMC: 4334126. DOI: 10.1126/scitranslmed.3009701. View

16.

Thurber G, Reiner T, Yang K, Kohler R, Weissleder R . Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol Cancer Ther. 2014; 13(4):986-95. PMC: 3981972. DOI: 10.1158/1535-7163.MCT-13-0801. View

17.

Jasnos L, Sawado T . Determining cell division symmetry through the dissection of dividing cells using single-cell expression analysis. Nat Protoc. 2014; 9(3):505-16. DOI: 10.1038/nprot.2014.032. View

18.

Amir E, Davis K, Tadmor M, Simonds E, Levine J, Bendall S . viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia. Nat Biotechnol. 2013; 31(6):545-52. PMC: 4076922. DOI: 10.1038/nbt.2594. View

19.

Piccirillo S, Colman S, Potter N, van Delft F, Lillis S, Carnicer M . Genetic and functional diversity of propagating cells in glioblastoma. Stem Cell Reports. 2014; 4(1):7-15. PMC: 4297869. DOI: 10.1016/j.stemcr.2014.11.003. View

20.

Treutlein B, Brownfield D, Wu A, Neff N, Mantalas G, Espinoza F . Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature. 2014; 509(7500):371-5. PMC: 4145853. DOI: 10.1038/nature13173. View