Characterization of a Thioredoxin-1 Gene from Taenia Solium and Its Encoding Product

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Jun 20

PMID 26090410

Citations 3

Authors

Lucia Jimenez

Oscar Rodriguez-Lima

Alicia Ochoa-Sanchez

Abraham Landa

Affiliations

Soon will be listed here.

Abstract

Taenia solium thioredoxin-1 gene (TsTrx-1) has a length of 771 bp with three exons and two introns. The core promoter gene presents two putative stress transcription factor binding sites, one putative TATA box, and a transcription start site (TSS). TsTrx-1 mRNA is expressed higher in larvae than in adult. This gene encodes a protein of 107 amino acids that presents the Trx active site (CGPC), the classical secondary structure of the thioredoxin fold, and the highest degree of identity with the Echinococcus granulosus Trx. A recombinant TsTrx-1 (rTsTrx-1) was produced in Escherichia coli with redox activity. Optimal activity for rTsTrx-1 was at pH 6.5 in the range of 15 to 25°C. The enzyme conserved activity for 3 h and lost it in 24 h at 37°C. rTsTrx-1 lost 50% activity after 1 h and lost activity completely in 24 h at temperatures higher than 55°C. Best storage temperature for rTsTrx-1 was at -70°C. It was inhibited by high concentrations of H₂O₂ and methylglyoxal (MG), but it was inhibited neither by NaCl nor by anti-rTsTrx-1 rabbit antibodies that strongly recognized a ~12 kDa band in extracts from several parasites. These TsTrx-1 properties open the opportunity to study its role in relationship T. solium-hosts.

Citing Articles

Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium.

Miranda-Blancas R, Rodriguez-Lima O, Garcia-Gutierrez P, Flores-Lopez R, Jimenez L, Zubillaga R FEBS Open Bio. 2024; 14(5):726-739.

PMID: 38514457 PMC: 11073501. DOI: 10.1002/2211-5463.13795.

Cloning, expression, purification, and kinetic characterization of mitochondrial thioredoxin (TsTrx2), cytosolic thioredoxin (TsTrx1), and glutaredoxin (TsGrx1) from Taenia solium.

Nava G, Maldonado G, Plancarte A Parasitol Res. 2019; 118(6):1785-1797.

PMID: 31062084 DOI: 10.1007/s00436-019-06336-4.

Molecular Cloning of a cDNA Encoding for Taenia solium TATA-Box Binding Protein 1 (TsTBP1) and Study of Its Interactions with the TATA-Box of Actin 5 and Typical 2-Cys Peroxiredoxin Genes.

Rodriguez-Lima O, Garcia-Gutierrez P, Jimenez L, Zarain-Herzberg A, Lazzarini R, Landa A PLoS One. 2015; 10(11):e0141818.

PMID: 26529408 PMC: 4631506. DOI: 10.1371/journal.pone.0141818.

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