A Transgenic Resource for Conditional Competitive Inhibition of Conserved Drosophila MicroRNAs

Overview

Journal Nat Commun

Specialty Biology

Date 2015 Jun 18

PMID 26081261

Citations 47

Authors

Tudor A Fulga

Elizabeth M McNeill

Richard Binari

Julia Yelick

Alexandra Blanche

Matthew Booker

Bruno R Steinkraus

Michael Schnall-Levin

Yong Zhao

Todd DeLuca

Fernando Bejarano

Zhe Han

Eric C Lai

Dennis P Wall

Norbert Perrimon

David Van Vactor

Affiliations

Soon will be listed here.

Abstract

Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4-8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes.

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