Protein Residue Linking in a Single Spectrum for Magic-angle Spinning NMR Assignment

Overview

Journal J Biomol NMR

Publisher Springer

Specialties Molecular Biology
Nuclear Medicine

Date 2015 Jun 17

PMID 26078089

Citations 14

Authors

Loren B Andreas

Jan Stanek

Tanguy Le Marchand

Andrea Bertarello

Diane Cala-De Paepe

Daniela Lalli

Magdalena Krejcikova

Camille Doyen

Carl Oster

Benno Knott

Sebastian Wegner

Frank Engelke

Isabella C Felli

Roberta Pierattelli

Nicholas E Dixon

Lyndon Emsley

Torsten Herrmann

Guido Pintacuda

Affiliations

Soon will be listed here.

Abstract

Here we introduce a new pulse sequence for resonance assignment that halves the number of data sets required for sequential linking by directly correlating sequential amide resonances in a single diagonal-free spectrum. The method is demonstrated with both microcrystalline and sedimented deuterated proteins spinning at 60 and 111 kHz, and a fully protonated microcrystalline protein spinning at 111 kHz, with as little as 0.5 mg protein sample. We find that amide signals have a low chance of ambiguous linkage, which is further improved by linking in both forward and backward directions. The spectra obtained are amenable to automated resonance assignment using general-purpose software such as UNIO-MATCH.

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