Detection of Brucella Abortus DNA in Aborted Goats and Sheep in Egypt by Real-time PCR

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2015 Jun 4

PMID 26036697

Citations 26

Authors

Gamal Wareth

Falk Melzer

Herbert Tomaso

Uwe Roesler

Heinrich Neubauer

Affiliations

Soon will be listed here.

Abstract

Background: Brucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and camels.

Results: In the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact.

Conclusion: This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. Information provided in this study is important for surveillance program, because eradication programs and vaccination strategies may have to be adapted accordingly.

Citing Articles

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Menshawy A, Vicente A, Hegazy Y, Djokic V, Hamdy M, Freddi L Microorganisms. 2025; 13(1).

PMID: 39858938 PMC: 11767844. DOI: 10.3390/microorganisms13010170.

Molecular Testing of Zoonotic Bacteria in Cattle, Sheep, and Goat Abortion Cases in Botswana.

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Genome-Wide Identification of Specific Genetic Loci Common to Sheep and Goat.

Liang Z, Yue X, Liu Y, Ye M, Zhong L, Luan Y Biomolecules. 2024; 14(6).

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New insights into the genetic predisposition of brucellosis and its effect on the gut and vaginal microbiota in goats.

Sallam A, Abou-Souliman I, Reyer H, Wimmers K, Rabee A Sci Rep. 2023; 13(1):20086.

PMID: 37973848 PMC: 10654701. DOI: 10.1038/s41598-023-46997-x.

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