Biodistribution and Function of Extracellular MiRNA-155 in Mice

Overview

Journal Sci Rep

Specialty Science

Date 2015 May 30

PMID 26024046

Citations 77

Authors

Shashi Bala

Timea Csak

Fatemeh Momen-Heravi

Dora Lippai

Karen Kodys

Donna Catalano

Abhishek Satishchandran

Victor Ambros

Gyongyi Szabo

Affiliations

Soon will be listed here.

Abstract

Circulating miRNAs can be found in extracellular vesicles (EV) and could be involved in intercellular communication. Here, we report the biodistribution of EV associated miR-155 using miR-155 KO mouse model. Administration of exosomes loaded with synthetic miR-155 mimic into miR-155 KO mice resulted in a rapid accumulation and clearance of miR-155 in the plasma with subsequent distribution in the liver, adipose tissue, lung, muscle and kidney (highest to lowest, respectively). miR-155 expression was detected in isolated hepatocytes and liver mononuclear cells of recipient KO mice suggesting its cellular uptake. In vitro, exosome-mediated restoration of miR-155 in Kupffer cells from miR-155 deficient mice augmented their LPS-induced MCP1 mRNA increase. The systemic delivery of wild type plasma to miR-155 KO mice also resulted in a rapid accumulation of miR-155 in the circulation and distribution to the liver and adipose tissue. In summary, our results demonstrate tissue biodistribution and biologic function of EV-associated miR-155.

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