Male Germ-like Cell Differentiation Potential of Human Umbilical Cord Wharton's Jelly-derived Mesenchymal Stem Cells in Co-culture with Human Placenta Cells in Presence of BMP4 and Retinoic Acid

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2015 May 29

PMID 26019794

Citations 15

Authors

Nahid Ataie Nejad

Fardin Amidi

Marziyeh Agha Hoseini

Karim Nayer Nia

Mehryar Habibi

Abdol Mohammad Kajbafzadeh

Zohreh Mazaheri

Nazila Yamini

Affiliations

Soon will be listed here.

Abstract

Objectives: Mesenchymal stem cells (MSCs) derived from Wharton's jelly (WJ-MSCs) are now much more appealing for cell-based infertility therapy. Hence, WJ-MSCs differentiation toward germ layer cells for cell therapy purposes is currently under intensive study.

Materials And Methods: MSCs were isolated from human Wharton's jelly and treated with BMP4, retinoic acid (RA) or co-cultured on human amniotic epithelial (HAE) and chorionic plate (HCP) placenta feeder cells. profile of POU5F1, Fragilis, Plzf, DDX4, Piwil2, Stra8, Dazl, β1- and α6-integrins (ITΒ1, ITA6) genes expression as germ cell markers were analyzed using RT-PCR and real-time PCR. Immunocytochemistry of surface markers were conducted.

Results: After 3 weeks treatment with different reagents and co-culture system, morphology of WJ-MSCs changed to shiny clusters and germ cell specific markers in mRNA were up-regulated in both placental feeder + RA and BMP4 + RA. Induction of hWJ-MSCs with BMP4 in presence of RA resulted in significant up-regulation (P≤0.05) of all germ cell specific genes (c-Kit; 2.84±0.59, DDX4; 1.69±0.39, Piwil2; 1.14±0.21, Dazl; 0.65±0.25, α6 integrin; 1.26±0.53, β1 integrins; 1.18±0.65) compared to control and placental feeder cells + RA. Our results indicated that HAE and HCP followed by RA treatment were involved in human germ cell development.

Conclusion: We demonstrated that under the right conditions, hWJ-MSCs have the ability to differentiate to germ cells and this provides an excellent pattern to study infertility cause and treatment.

Citing Articles

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Current Progress in Stem Cell Therapy for Male Infertility.

Adriansyah R, Margiana R, Supardi S, Narulita P Stem Cell Rev Rep. 2023; 19(7):2073-2093.

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Differentiation of Human Wharton Jelly Mesenchymal Stem Cells into Germ-Like Cells; emphasis on evaluation of Germ-long non-coding RNAs.

Ghasemi S, Shafiee M, Ferns G, Tavakol-Afshari J, Saeedi M, Raji S Mol Biol Rep. 2022; 49(12):11901-11912.

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Strategies for Mammalian Mesenchymal Stem Cells Differentiation into Primordial Germ Cell-Like Cells: A Review.

Fayezi S, Fayyazpour P, Norouzi Z, Mehdizadeh A Cell J. 2022; 24(8):434-441.

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