The Biphasic Effects of Oxidized-low Density Lipoprotein on the Vasculogenic Function of Endothelial Progenitor Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 May 29

PMID 26017136

Citations 13

Authors

Feng-Yen Lin

Nai-Wen Tsao

Chun-Ming Shih

Yi-Wen Lin

Jong-Shiua Yeh

Jaw-Wen Chen

Hironori Nakagami

Ryuichi Morishita

Tatsuya Sawamura

Chun-Yao Huang

Affiliations

Soon will be listed here.

Abstract

Late-outgrowth endothelial progenitor cells (EPCs) are stress-resistant and responsible for reparative functions in the cardiovascular system. Oxidized-LDL (oxLDL) plays a critical role in cardiovascular disease pathogenesis. However, it is largely unknown what the impacts of oxLDL are on late-outgrowth EPCs. This study aimed to investigate the concentration-related effects of oxLDL on EPC functions and related angiogenesis, in vitro and in vivo. In this study, early and late-outgrowth EPCs were generated from circulating human mononuclear cells. oxLDL may regulate EPC vasculogenic function via the lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1). Lower concentrations (5 μg/mL) of oxLDL can potentiate EPC tube formation in vitro and in vivo by activating eNOS mechanisms, which are mediated by p38 MAPK- and SAPK/JNK-related pathways. Higher concentrations of oxLDL (10-50 μg/mL) impaired EPC function via the activation of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase pathways and consequent inhibition of eNOS activity, which could be reversed by anti-oxidants (diphenylene iodonium and apocynin) and gp91phox siRNA. In conclusion, oxLDL has concentration-dependent biphasic effects on human late-outgrowth EPC tube formation in vitro and in vivo.

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