Brimonidine Reduces TGF-beta-induced Extracellular Matrix Synthesis in Human Tenon's Fibroblasts

Overview

Journal BMC Ophthalmol

Publisher Biomed Central

Specialty Ophthalmology

Date 2015 May 29

PMID 26017119

Citations 7

Authors

Samin Hong

Sueng-Han Han

Chan Yun Kim

Kang Yoon Kim

Yoo Kyung Song

Gong Je Seong

Affiliations

Soon will be listed here.

Abstract

Background: Brimonidine is a highly selective α2 adrenergic agonist that has been widely used in anti-glaucoma eyedrops. The aim of this study was to investigate its putative anti-fibrotic role in the fibrosis caused by activated Tenon's fibroblasts.

Methods: Primary cultured human Tenon's fibroblasts were exposed to 2.0 ng/mL of transforming growth factor-β1 (TGF-β1) for up to 48 h. In the presence of various concentrations of brimonidine (from 0.0 to 10.0 μM), the expression levels of fibronectin, collagen types I and III, and β-actin were determined by Western immunoblots. The expression of phosphorylated SMAD2/3 (p-SMAD2/3) was then evaluated using immunofluorescence.

Results: TGF-β1 significantly increased the synthesis of fibronectin and collagens in human Tenon's fibroblasts; however brimonidine treatment distinctly attenuated the TGF-β1-induced production of extracellular matrix (ECM) proteins. TGF-β1 also changed the cellular morphology to be plump, while brimonidine treatment returned the cells to a spindle shape, similar to control fibroblasts. Regarding p-SMAD2/3, brimonidine treatment did not show any apparent changes in its expression.

Conclusions: Our data revealed that brimonidine reduces TGF-β-induced ECM synthesis in human Tenon's fibroblasts in vitro. This finding implies that topical administration of brimonidine may be helpful in reducing the fibrosis caused by the long-term use of topical anti-glaucoma medications.

Citing Articles

An α2-Adrenergic Agonist, Brimonidine, Beneficially Affects the TGF-β2-Treated Cellular Properties in an In Vitro Culture Model.

Watanabe M, Sato T, Tsugeno Y, Higashide M, Furuhashi M, Umetsu A Bioengineering (Basel). 2022; 9(7).

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Elucidating the Therapeutic Potential of Cell-Penetrating Peptides in Human Tenon Fibroblast Cells.

Chatterjee A, Ansar S, Gopal D, Vetrivel U, George R, Narayanan J ACS Omega. 2022; 7(19):16536-16546.

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LncRNA NR_003923 promotes cell proliferation, migration, fibrosis, and autophagy via the miR-760/miR-215-3p/IL22RA1 axis in human Tenon's capsule fibroblasts.

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Acute Hyperthermia Inhibits TGF-β1-induced Cardiac Fibroblast Activation via Suppression of Akt Signaling.

Narikawa M, Umemura M, Tanaka R, Fujita T, Yokoyama U, Ishigami T Sci Rep. 2018; 8(1):6277.

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Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2, 3 signal transduction .

Feng Z, Zhang X, Zhao J, Ma J Int J Ophthalmol. 2017; 10(12):1805-1811.

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