In Vitro Methods for Hazard Assessment of Industrial Chemicals - Opportunities and Challenges

Overview

Journal Front Pharmacol

Date 2015 May 23

PMID 25999858

Citations 5

Authors

Chin Lin Wong

Sussan Ghassabian

Maree T Smith

Ai-Leen Lam

Affiliations

Soon will be listed here.

Abstract

Allergic contact dermatitis (ACD) is a delayed-type hypersensitivity immune reaction mediated by T-lymphocytes as a result of repeated exposure of an allergen primarily on skin. ACD accounts for up to 95% of occupational skin diseases, with epoxy resins implicated as one of the most common causes of ACD. Efficient high-throughput in vitro screening for accurate identification of compounds and materials that may pose hazardous risks in the workplace is crucial. At present, the murine local lymph node assay is the 'method of choice' for predicting the sensitizing potency of contact allergens. As the 3Rs principles of reduction, refinement, and replacement in animal testing has gained political and economic momentum, several in vitro screening methods have been developed for identifying potential contact allergens. To date, these latter methods have been utilized primarily to assess the skin sensitizing potential of the chemical components of cosmetic products with scant research attention as to the applicability of these methods to industrial chemicals, particularly epoxy resins. Herein we review the currently utilized in vitro methods and identify the knowledge gaps with regard to assessing the generalizability of in vitro screening methods for assessing the skin sensitizing potential of industrial chemicals.

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References

Wanner R, Sonnenburg A, Quatchadze M, Schreiner M, Peiser M, Zuberbier T . Classification of sensitizing and irritative potential in a combined in-vitro assay. Toxicol Appl Pharmacol. 2010; 245(2):211-8. DOI: 10.1016/j.taap.2010.02.019. View

Emter R, Ellis G, Natsch A . Performance of a novel keratinocyte-based reporter cell line to screen skin sensitizers in vitro. Toxicol Appl Pharmacol. 2010; 245(3):281-90. DOI: 10.1016/j.taap.2010.03.009. View

Natsch A, Emter R, Ellis G . Filling the concept with data: integrating data from different in vitro and in silico assays on skin sensitizers to explore the battery approach for animal-free skin sensitization testing. Toxicol Sci. 2008; 107(1):106-21. DOI: 10.1093/toxsci/kfn204. View

Niklasson I, Broo K, Jonsson C, Luthman K, Karlberg A . Reduced sensitizing capacity of epoxy resin systems: a structure-activity relationship study. Chem Res Toxicol. 2009; 22(11):1787-94. DOI: 10.1021/tx900193s. View

Koss A, Lucero G, Koziner B . Granulocyte-colony stimulating factor, granulocyte-macrophage colony stimulating factor and interleukin 4 induce differentiation in the U-937 human monocytic leukemia cell line. Leuk Lymphoma. 1996; 22(1-2):163-71,follow.186,color plate XIV-V. DOI: 10.3109/10428199609051744. View

Roberts D, Natsch A . High throughput kinetic profiling approach for covalent binding to peptides: application to skin sensitization potency of Michael acceptor electrophiles. Chem Res Toxicol. 2009; 22(3):592-603. DOI: 10.1021/tx800431x. View

Carlson R, Boyd K, Webb D . The revision of the Declaration of Helsinki: past, present and future. Br J Clin Pharmacol. 2004; 57(6):695-713. PMC: 1884510. DOI: 10.1111/j.1365-2125.2004.02103.x. View

Ade N, Martinozzi-Teissier S, Pallardy M, Rousset F . Activation of U937 cells by contact sensitizers: CD86 expression is independent of apoptosis. J Immunotoxicol. 2008; 3(4):189-97. DOI: 10.1080/15476910600978038. View

Aeby P, Ashikaga T, Bessou-Touya S, Schepky A, Gerberick F, Kern P . Identifying and characterizing chemical skin sensitizers without animal testing: Colipa's research and method development program. Toxicol In Vitro. 2010; 24(6):1465-73. DOI: 10.1016/j.tiv.2010.07.005. View

10.

Cashman M, Reutemann P, Ehrlich A . Contact dermatitis in the United States: epidemiology, economic impact, and workplace prevention. Dermatol Clin. 2011; 30(1):87-98, viii. DOI: 10.1016/j.det.2011.08.004. View

11.

Yoshida Y, Sakaguchi H, Ito Y, Okuda M, Suzuki H . Evaluation of the skin sensitization potential of chemicals using expression of co-stimulatory molecules, CD54 and CD86, on the naive THP-1 cell line. Toxicol In Vitro. 2003; 17(2):221-8. DOI: 10.1016/s0887-2333(03)00006-7. View

12.

Merckel F, Bernard G, Mutschler J, Gimenez-Arnau E, Gerberick G, Lepoittevin J . Effect of a microemulsion system on hapten-peptide reactivity studies: examples of hydroxycitronellal and citral, fragrance skin sensitizers, with glutathione. Chem Res Toxicol. 2010; 23(9):1433-41. DOI: 10.1021/tx100043b. View

13.

Jowsey I, Basketter D, Westmoreland C, Kimber I . A future approach to measuring relative skin sensitising potency: a proposal. J Appl Toxicol. 2006; 26(4):341-50. DOI: 10.1002/jat.1146. View

14.

Richter A, Schmucker S, Esser P, Traska V, Weber V, Dietz L . Human T cell priming assay (hTCPA) for the identification of contact allergens based on naive T cells and DC--IFN-γ and TNF-α readout. Toxicol In Vitro. 2012; 27(3):1180-5. DOI: 10.1016/j.tiv.2012.08.007. View

15.

Kaplan D, Igyarto B, Gaspari A . Early immune events in the induction of allergic contact dermatitis. Nat Rev Immunol. 2012; 12(2):114-24. PMC: 3578582. DOI: 10.1038/nri3150. View

16.

Troutman J, Foertsch L, Kern P, Jian Dai H, Quijano M, Dobson R . The incorporation of lysine into the peroxidase peptide reactivity assay for skin sensitization assessments. Toxicol Sci. 2011; 122(2):422-36. DOI: 10.1093/toxsci/kfr101. View

17.

Bennett M, Lowney A, Bourke J . A study of occupational contact dermatitis in the pharmaceutical industry. Br J Dermatol. 2015; 174(3):654-6. DOI: 10.1111/bjd.14177. View

18.

Gamer A, Nies E, Vohr H . Local lymph node assay (LLNA): comparison of different protocols by testing skin-sensitizing epoxy resin system components. Regul Toxicol Pharmacol. 2008; 52(3):290-8. DOI: 10.1016/j.yrtph.2008.08.018. View

19.

Basketter D, Maxwell G . In vitro approaches to the identification and characterization of skin sensitizers. Cutan Ocul Toxicol. 2007; 26(4):359-73. DOI: 10.1080/15569520701622993. View

20.

McKim Jr J, Keller 3rd D, Gorski J . An in vitro method for detecting chemical sensitization using human reconstructed skin models and its applicability to cosmetic, pharmaceutical, and medical device safety testing. Cutan Ocul Toxicol. 2012; 31(4):292-305. DOI: 10.3109/15569527.2012.667031. View