Structural and Functional Studies of Membrane Remodeling Machines

Overview

Journal Methods Cell Biol

Specialty Cell Biology

Date 2015 May 23

PMID 25997348

Citations 6

Authors

Raghav Kalia

Nathaniel Talledge

Adam Frost

Affiliations

Soon will be listed here.

Abstract

Building cells from their component parts will hinge upon our ability to reconstitute biochemical compartmentalization and exchange between membrane-delimited organelles. By contrast with our understanding of other cellular events, the mechanisms that govern membrane trafficking has lagged because the presence of phospholipid bilayers complicates the use of standard methods. This chapter describes in vitro methods for purifying, reconstituting, and visualizing membrane remodeling activities directly by electron cryomicroscopy.

Citing Articles

Differential impact of ubiquitous and muscle dynamin 2 isoforms in muscle physiology and centronuclear myopathy.

Gomez-Oca R, Edelweiss E, Djeddi S, Gerbier M, Massana-Munoz X, Oulad-Abdelghani M Nat Commun. 2022; 13(1):6849.

PMID: 36369230 PMC: 9652393. DOI: 10.1038/s41467-022-34490-4.

BIN1 modulation in vivo rescues dynamin-related myopathy.

Lionello V, Kretz C, Edelweiss E, Crucifix C, Gomez-Oca R, Messaddeq N Proc Natl Acad Sci U S A. 2022; 119(9).

PMID: 35217605 PMC: 8892365. DOI: 10.1073/pnas.2109576119.

Open and cut: allosteric motion and membrane fission by dynamin superfamily proteins.

Kalia R, Frost A Mol Biol Cell. 2019; 30(17):2097-2104.

PMID: 31365329 PMC: 6743466. DOI: 10.1091/mbc.E16-10-0709.

Structural basis of mitochondrial receptor binding and constriction by DRP1.

Kalia R, Wang R, Yusuf A, Thomas P, Agard D, Shaw J Nature. 2018; 558(7710):401-405.

PMID: 29899447 PMC: 6120343. DOI: 10.1038/s41586-018-0211-2.

Amphiphysin (BIN1) negatively regulates dynamin 2 for normal muscle maturation.

Cowling B, Prokic I, Tasfaout H, Rabai A, Humbert F, Rinaldi B J Clin Invest. 2017; 127(12):4477-4487.

PMID: 29130937 PMC: 5707152. DOI: 10.1172/JCI90542.

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