Extracellular Vesicles Such As Prostate Cancer Cell Fragments As a Fluid Biopsy for Prostate Cancer

Overview

Journal Prostate Cancer Prostatic Dis

Specialties Oncology
Urology

Date 2015 May 13

PMID 25964141

Citations 4

Authors

S I Brett

Y Kim

C N Biggs

J L Chin

H S Leong

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are cell-derived vesicles generated through a process of cell membrane shedding or storage vesicle release, as occurs during apoptosis, necrosis or exocytosis. Initially perceived as cellular by-products or 'dust' of insignificant biological importance, recent research has shed light on the role of EVs as mediators of intercellular communication, blood coagulation and disease progression. The prostate is a source of EVs and their abundance in complex biological fluids such as plasma, serum and urine make them compelling entities for a 'fluid biopsy'. As such, prostate cancer cell fragments (PCCF) are EVs generated by the tumor resident within the prostate and are also present in blood, expressing a portion of biomarkers representative of the primary tumor. High-throughput analytical techniques to determine biomarker expression on EVs is the last hurdle towards translating the full potential of prostate EVs for clinical use. We describe current state-of-the-art methods for the analysis of prostate-derived EVs in patient fluids such as plasma and the challenges that lie ahead in this emerging field of translational research.

Citing Articles

Cell-Derived Vesicles with Increased Stability and On-Demand Functionality by Equipping Their Membrane with a Cross-Linkable Copolymer.

Huang X, Hurlimann D, Spanke H, Wu D, Skowicki M, Dinu I Adv Healthc Mater. 2022; 11(23):e2202100.

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AFM-compatible microfluidic platform for affinity-based capture and nanomechanical characterization of circulating tumor cells.

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Clinical significance of STEAP1 extracellular vesicles in prostate cancer.

Khanna K, Salmond N, Lynn K, Leong H, Williams K Prostate Cancer Prostatic Dis. 2021; 24(3):802-811.

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Plasma Exosome Profiling of Cancer Patients by a Next Generation Systems Biology Approach.

Domenyuk V, Zhong Z, Stark A, Xiao N, ONeill H, Wei X Sci Rep. 2017; 7:42741.

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References

Lee T, DAsti E, Magnus N, Al-Nedawi K, Meehan B, Rak J . Microvesicles as mediators of intercellular communication in cancer--the emerging science of cellular 'debris'. Semin Immunopathol. 2011; 33(5):455-67. DOI: 10.1007/s00281-011-0250-3. View

Sardana G, Diamandis E . Biomarkers for the diagnosis of new and recurrent prostate cancer. Biomark Med. 2012; 6(5):587-96. DOI: 10.2217/bmm.12.72. View

Podor T, Singh D, Chindemi P, Foulon D, McKelvie R, Weitz J . Vimentin exposed on activated platelets and platelet microparticles localizes vitronectin and plasminogen activator inhibitor complexes on their surface. J Biol Chem. 2001; 277(9):7529-39. DOI: 10.1074/jbc.M109675200. View

Lowes L, Lock M, Rodrigues G, DSouza D, Bauman G, Ahmad B . Circulating tumour cells in prostate cancer patients receiving salvage radiotherapy. Clin Transl Oncol. 2012; 14(2):150-6. DOI: 10.1007/s12094-012-0775-5. View

Duijvesz D, Burnum-Johnson K, Gritsenko M, Hoogland A, den Berg M, Willemsen R . Proteomic profiling of exosomes leads to the identification of novel biomarkers for prostate cancer. PLoS One. 2014; 8(12):e82589. PMC: 3876995. DOI: 10.1371/journal.pone.0082589. View

Heijnen H, Schiel A, Fijnheer R, Geuze H, Sixma J . Activated platelets release two types of membrane vesicles: microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules. Blood. 1999; 94(11):3791-9. View

de Bono J, Scher H, Montgomery R, Parker C, Miller M, Tissing H . Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin Cancer Res. 2008; 14(19):6302-9. DOI: 10.1158/1078-0432.CCR-08-0872. View

Ravichandran K . Find-me and eat-me signals in apoptotic cell clearance: progress and conundrums. J Exp Med. 2010; 207(9):1807-17. PMC: 2931173. DOI: 10.1084/jem.20101157. View

Ihlaseh-Catalano S, Drigo S, de Jesus C, Domingues M, Trindade Filho J, de Camargo J . STEAP1 protein overexpression is an independent marker for biochemical recurrence in prostate carcinoma. Histopathology. 2013; 63(5):678-85. DOI: 10.1111/his.12226. View

10.

Adams D, Stefansson S, Haudenschild C, Martin S, Charpentier M, Chumsri S . Cytometric characterization of circulating tumor cells captured by microfiltration and their correlation to the CellSearch(®) CTC test. Cytometry A. 2014; 87(2):137-44. DOI: 10.1002/cyto.a.22613. View

11.

Mesri M, Altieri D . Endothelial cell activation by leukocyte microparticles. J Immunol. 1998; 161(8):4382-7. View

12.

Selth L, Townley S, Gillis J, Ochnik A, Murti K, Macfarlane R . Discovery of circulating microRNAs associated with human prostate cancer using a mouse model of disease. Int J Cancer. 2011; 131(3):652-61. DOI: 10.1002/ijc.26405. View

13.

Itoh T, Ito Y, Ohtsuki Y, Ando M, Tsukamasa Y, Yamada N . Microvesicles released from hormone-refractory prostate cancer cells facilitate mouse pre-osteoblast differentiation. J Mol Histol. 2012; 43(5):509-15. PMC: 3460166. DOI: 10.1007/s10735-012-9415-1. View

14.

Doyen J, Alix-Panabieres C, Hofman P, Parks S, Chamorey E, Naman H . Circulating tumor cells in prostate cancer: a potential surrogate marker of survival. Crit Rev Oncol Hematol. 2011; 81(3):241-56. DOI: 10.1016/j.critrevonc.2011.05.004. View

15.

Hargett L, Bauer N . On the origin of microparticles: From "platelet dust" to mediators of intercellular communication. Pulm Circ. 2013; 3(2):329-40. PMC: 3757826. DOI: 10.4103/2045-8932.114760. View

16.

Mrvar-Brecko A, Sustar V, Jansa V, Stukelj R, Jansa R, Mujagic E . Isolated microvesicles from peripheral blood and body fluids as observed by scanning electron microscope. Blood Cells Mol Dis. 2010; 44(4):307-12. DOI: 10.1016/j.bcmd.2010.02.003. View

17.

Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee J, Lotvall J . Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007; 9(6):654-9. DOI: 10.1038/ncb1596. View

18.

Xie Y, Bai O, Yuan J, Chibbar R, Slattery K, Wei Y . Tumor apoptotic bodies inhibit CTL responses and antitumor immunity via membrane-bound transforming growth factor-beta1 inducing CD8+ T-cell anergy and CD4+ Tr1 cell responses. Cancer Res. 2009; 69(19):7756-66. DOI: 10.1158/0008-5472.CAN-09-0496. View

19.

Mizutani K, Terazawa R, Kameyama K, Kato T, Horie K, Tsuchiya T . Isolation of prostate cancer-related exosomes. Anticancer Res. 2014; 34(7):3419-23. View

20.

George J, Thoi L, McManus L, Reimann T . Isolation of human platelet membrane microparticles from plasma and serum. Blood. 1982; 60(4):834-40. View