Cyclooxygenase-2--An Imperative Prognostic Biomarker in Oral Squamous Cell Carcinoma--An Immunohistochemical Study

Overview

Journal Pathol Oncol Res

Specialty Oncology

Date 2015 May 13

PMID 25962348

Citations 15

Authors

Aditi Amit Byatnal

Amit Byatnal

Subhalakshmi Sen

Vasudev Guddattu

Monica Charlotte Solomon

Affiliations

Soon will be listed here.

Abstract

Oral squamous cell carcinoma (OSCC) is the most common head and neck squamous cell carcinoma (HNSCC) with metastasis and tumor recurrence resulting in 90 % of cancer associated mortality. COX-2, an inflammatory biomarker, has been shown to play a significant role in tumorigenesis of OSCC. To study the expression of COX-2 in OSCC by immunohistochemistry and investigate its association with the clinicopathological parameters including patient survival. A cross sectional study was carried out in 75 histologically confirmed cases of OSCC. COX-2 expression was evaluated by indirect streptavidin biotin method. The expression was semi-quantitatively assessed using established criteria. The expression profile of COX-2 was correlated with the clinicopathological details like tumor size, regional lymphnode metastasis, distant metastasis, clinical stage, local recurrence of tumor, histological grade, and survival of patient. Chi square and Kaplan Meier statistical tests were applied for assessing this association. COX-2 expression was absent in normal oral mucosa. Over expression of COX-2 was seen in 58 out of 75 specimens of OSCC. Overexpression of COX-2 was significantly associated with the lymphnode involvement, histological grade, local recurrence of tumor and patient survival. COX-2 expression represents an important biomarker of prognostic significance that may be used to identify a subset of patients at high risk and to predict patient survival.

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References

Kujubu D, Fletcher B, Varnum B, Lim R, Herschman H . TIS10, a phorbol ester tumor promoter-inducible mRNA from Swiss 3T3 cells, encodes a novel prostaglandin synthase/cyclooxygenase homologue. J Biol Chem. 1991; 266(20):12866-72. View

Greenhough A, Smartt H, Moore A, Roberts H, Williams A, Paraskeva C . The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumour microenvironment. Carcinogenesis. 2009; 30(3):377-86. DOI: 10.1093/carcin/bgp014. View

Tsujii M, Kawano S, DuBois R . Cyclooxygenase-2 expression in human colon cancer cells increases metastatic potential. Proc Natl Acad Sci U S A. 1997; 94(7):3336-40. PMC: 20370. DOI: 10.1073/pnas.94.7.3336. View

Kirschenbaum A, Liotta D, Yao S, Liu X, Klausner A, Unger P . Immunohistochemical localization of cyclooxygenase-1 and cyclooxygenase-2 in the human fetal and adult male reproductive tracts. J Clin Endocrinol Metab. 2000; 85(9):3436-41. DOI: 10.1210/jcem.85.9.6780. View

Hanahan D, Folkman J . Patterns and emerging mechanisms of the angiogenic switch during tumorigenesis. Cell. 1996; 86(3):353-64. DOI: 10.1016/s0092-8674(00)80108-7. View

Dormond O, Foletti A, Paroz C, Ruegg C . NSAIDs inhibit alpha V beta 3 integrin-mediated and Cdc42/Rac-dependent endothelial-cell spreading, migration and angiogenesis. Nat Med. 2001; 7(9):1041-7. DOI: 10.1038/nm0901-1041. View

Sano H, Kawahito Y, Wilder R, Hashiramoto A, Mukai S, Asai K . Expression of cyclooxygenase-1 and -2 in human colorectal cancer. Cancer Res. 1995; 55(17):3785-9. View

Hamada G, Bos A, Kasuga H, Hirayama T . Comparative epidemiology of oral cancer in Brazil and India. Tokai J Exp Clin Med. 1991; 16(1):63-72. View

Li W, Ding Y, Li Z, Zhang J . [Expression of COX-2 and VEGF-C in squamous cell carcinoma of the tongue and its correlation to lymph node metastasis]. Nan Fang Yi Ke Da Xue Xue Bao. 2008; 28(2):180-3. View

10.

Grivennikov S, Greten F, Karin M . Immunity, inflammation, and cancer. Cell. 2010; 140(6):883-99. PMC: 2866629. DOI: 10.1016/j.cell.2010.01.025. View

11.

Lipari L, Mauro A, Gallina S, Tortorici S, Buscemi M, Tete S . Expression of gelatinases (MMP-2, MMP-9) and cyclooxygenases (COX-1, COX-2) in some benign salivary gland tumors. Int J Immunopathol Pharmacol. 2012; 25(1):107-15. DOI: 10.1177/039463201202500113. View

12.

Jemal A, Thomas A, Murray T, Thun M . Cancer statistics, 2002. CA Cancer J Clin. 2002; 52(1):23-47. DOI: 10.3322/canjclin.52.1.23. View

13.

Gallo O, Masini E, Bianchi B, Bruschini L, Paglierani M, Franchi A . Prognostic significance of cyclooxygenase-2 pathway and angiogenesis in head and neck squamous cell carcinoma. Hum Pathol. 2002; 33(7):708-14. DOI: 10.1053/hupa.2002.125376. View

14.

Birchmeier C, Birchmeier W, Gherardi E, Vande Woude G . Met, metastasis, motility and more. Nat Rev Mol Cell Biol. 2003; 4(12):915-25. DOI: 10.1038/nrm1261. View

15.

Tucker O, Dannenberg A, Yang E, Zhang F, Teng L, Daly J . Cyclooxygenase-2 expression is up-regulated in human pancreatic cancer. Cancer Res. 1999; 59(5):987-90. View

16.

Itoh S, Matsui K, Furuta I, Takano Y . Immunohistochemical study on overexpression of cyclooxygenase-2 in squamous cell carcinoma of the oral cavity: its importance as a prognostic predictor. Oral Oncol. 2003; 39(8):829-35. DOI: 10.1016/s1368-8375(03)00105-2. View

17.

Svensson C, Yaksh T . The spinal phospholipase-cyclooxygenase-prostanoid cascade in nociceptive processing. Annu Rev Pharmacol Toxicol. 2002; 42:553-83. DOI: 10.1146/annurev.pharmtox.42.092401.143905. View

18.

Wang Y, Wu M, Yang A, Zhang W, Sun J, Liu T . COX-2 Gene increases tongue cancer cell proliferation and invasion through VEGF-C pathway. Med Oncol. 2010; 28 Suppl 1:S360-6. DOI: 10.1007/s12032-010-9737-3. View

19.

Fujiwaki R, Iida K, Kanasaki H, Ozaki T, Hata K, Miyazaki K . Cyclooxygenase-2 expression in endometrial cancer: correlation with microvessel count and expression of vascular endothelial growth factor and thymidine phosphorylase. Hum Pathol. 2002; 33(2):213-9. DOI: 10.1053/hupa.2002.31292. View

20.

Yoshimura R, Sano H, Masuda C, Kawamura M, Tsubouchi Y, Chargui J . Expression of cyclooxygenase-2 in prostate carcinoma. Cancer. 2000; 89(3):589-96. View