Autophagy in Anti-apoptotic Effect of Augmenter of Liver Regeneration in HepG2 Cells

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2015 May 9

PMID 25954098

Citations 3

Authors

Hong-Bo Shi

Hai-Qing Sun

Hong-Lin Shi

Feng Ren

Yu Chen

De-Xi Chen

Jin-Li Lou

Zhong-Ping Duan

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the role of autophagy in the anti-apoptotic effect of augmenter of liver regeneration (ALR).

Methods: Autophagy was induced through serum deprivation. An ALR-expressing plasmid was transfected into HepG2 cells, and autophagic flux was determined using fluorescence microscopy, electron microscopy, Western blot and quantitative polymerase chain reaction (qPCR) assays. After ALR-expressing plasmid transfection, an autophagy inhibitor [3-methyladenine (3-MA)] was added to HepG2 cells, and apoptosis was observed using fluorescence microscopy and flow cytometry.

Results: Autophagy was activated in HepG2 cells, peaking at 24 h after serum deprivation. Microtubule-associated protein light chain three-II levels were higher in HepG2 cells treated with ALR than in control cells, fluorescence microscopy, electron microscopy and qPCR studies showed the similar trend, and p62 levels showed the opposite trend, which indicated that ALR may play an important role in increasing autophagy flux. The numbers of apoptotic cells were substantially higher in HepG2 cells treated with both ALR and 3-MA than in cells treated with ALR alone. Therefore, the protective effect of ALR was significantly attenuated or abolished when autophagy was inhibited, indicating that the anti-apoptotic effect of ALR may be related to autophagy.

Conclusion: ALR protects cells from apoptosis partly through increased autophagy in HepG2 cells and may be valuable as a new therapeutic treatment for liver disease.

Citing Articles

Size of portally deprived liver lobe after portal vein ligation and additional partial hepatectomy: Result of balancing proliferation and apoptosis.

Wei W, Hua C, Zhang T, Dirsch O, Gremse F, Homeyer A Sci Rep. 2020; 10(1):4893.

PMID: 32184404 PMC: 7078252. DOI: 10.1038/s41598-020-60310-0.

Attenuated lipotoxicity and apoptosis is linked to exogenous and endogenous augmenter of liver regeneration by different pathways.

Weiss T, Lupke M, Ibrahim S, Buechler C, Lorenz J, Ruemmele P PLoS One. 2017; 12(9):e0184282.

PMID: 28877220 PMC: 5587239. DOI: 10.1371/journal.pone.0184282.

Augmenter of liver regeneration protects against carbon tetrachloride-induced liver injury by promoting autophagy in mice.

Shi H, Han W, Shi H, Ren F, Chen D, Chen Y Oncotarget. 2017; 8(8):12637-12648.

PMID: 28061452 PMC: 5355041. DOI: 10.18632/oncotarget.14478.

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