In Vitro Study of Surface Modified Poly(ethylene Glycol)-Impregnated Sintered Bovine Bone Scaffolds on Human Fibroblast Cells

Overview

Journal Sci Rep

Specialty Science

Date 2015 May 8

PMID 25950377

Citations 10

Authors

Sumit Pramanik

Forough Ataollahi

Belinda Pingguan-Murphy

Azim Ataollahi Oshkour

Noor Azuan Abu Osman

Affiliations

Soon will be listed here.

Abstract

Scaffold design from xenogeneic bone has the potential for tissue engineering (TE). However, major difficulties impede this potential, such as the wide range of properties in natural bone. In this study, sintered cortical bones from different parts of a bovine-femur impregnated with biodegradable poly(ethylene glycol) (PEG) binder by liquid phase adsorption were investigated. Flexural mechanical properties of the PEG-treated scaffolds showed that the scaffold is stiffer and stronger at a sintering condition of 1000°C compared with 900°C. In vitro cytotoxicity of the scaffolds evaluated by Alamar Blue assay and microscopic tests on human fibroblast cells is better at 1000°C compared with that at 900°C. Furthermore, in vitro biocompatibility and flexural property of scaffolds derived from different parts of a femur depend on morphology and heat-treatment condition. Therefore, the fabricated scaffolds from the distal and proximal parts at 1000°C are potential candidates for hard and soft TE applications, respectively.

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