Improvement of the Vitrification Method Suppressing the Disturbance of Meiotic Spindle and Chromosome Systems in Mature Oocytes

Overview

Journal Dev Reprod

Specialty Biology

Date 2015 May 8

PMID 25949179

Citations 3

Authors

Yun Jin Jung

Yong-Pil Cheon

Affiliations

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Abstract

Vitrification method is widely used in oocyte cryopreservation for IVF but the birth rates are lower than that of the fresh oocyte. One of the known main reasons is structural instability of meiotic spindle and chromosome systems of mature oocyte. To get the best way for keeping competence of matured oocytes, we studied the best conditions for vitrification focused on equilibration times. The mature oocytes were underwent vitrification with current popular method and analyzed the survival rates, microtubule stability and DNA integrity. The survival rates of recovered oocyte are almost same between groups and are more than 93%. The structural configuration of meiotic spindle was well kept in 10 min equilibration group and the stability rate was almost same with that of control. The chromosomal breakdown was observed in all experimental groups, but the chromosomal stability was higher in 10 min equilibration group than the other groups. The 10 min equilibration group showed best condition compared with the other groups. Based on these results, the equilibration time is one of the key factors in successful keeping for competence of mature oocyte. Although, more fine analysis about the effects of physical stress on oocyte during vitrification is needed to define the optimal condition, it is suggested that the optimal equilibration time to get competent oocyte in mouse is 10 min. Information acquired this study may provide insight into intracellular structural events occurring in human oocytes after vitrification and application for cryopreservation of human oocyte.

Citing Articles

Comparing the effects of a commercial and a prototype vitrification medium on meiotic spindle morphology and survival rate of mouse oocytes.

Viana I, Vireque A, Navarro P JBRA Assist Reprod. 2022; 26(3):500-507.

PMID: 35261222 PMC: 9355432. DOI: 10.5935/1518-0557.20210117.

Impact of prolonged oocyte incubation time before vitrification on oocyte survival, embryo formation, and embryo quality in mice.

Karami A, Bakhtiari M, Azadbakht M, Ghorbani R, Khazaei M, Rezaei M In Vitro Cell Dev Biol Anim. 2017; 53(6):525-531.

PMID: 28160207 DOI: 10.1007/s11626-017-0130-9.

Long Cut Straw Provides Stable the Rates of Survival, Pregnancy and Live Birth for Vitrification of Human Blasotcysts.

Lee J, Cha J, Shin S, Kim Y, Lee S, Cha H Dev Reprod. 2016; 20(3):219-225.

PMID: 27796003 PMC: 5078147. DOI: 10.12717/DR.2016.20.3.219.

References

Vincent C, Garnier V, Heyman Y, Renard J . Solvent effects on cytoskeletal organization and in-vivo survival after freezing of rabbit oocytes. J Reprod Fertil. 1989; 87(2):809-20. DOI: 10.1530/jrf.0.0870809. View

Yoon T, Kim T, Park S, Hong S, Ko J, Chung H . Live births after vitrification of oocytes in a stimulated in vitro fertilization-embryo transfer program. Fertil Steril. 2003; 79(6):1323-6. DOI: 10.1016/s0015-0282(03)00258-9. View

Lucena E, Bernal D, Lucena C, Rojas A, Moran A, Lucena A . Successful ongoing pregnancies after vitrification of oocytes. Fertil Steril. 2006; 85(1):108-11. DOI: 10.1016/j.fertnstert.2005.09.013. View

Borini A, Bonu M, Coticchio G, Bianchi V, Cattoli M, Flamigni C . Pregnancies and births after oocyte cryopreservation. Fertil Steril. 2004; 82(3):601-5. DOI: 10.1016/j.fertnstert.2004.04.025. View

Veeck L . Does the developmental stage at freeze impact on clinical results post-thaw?. Reprod Biomed Online. 2003; 6(3):367-74. DOI: 10.1016/s1472-6483(10)61859-7. View

Mandelbaum J, Belaisch-Allart J, Junca A, Antoine J, Plachot M, Alvarez S . Cryopreservation in human assisted reproduction is now routine for embryos but remains a research procedure for oocytes. Hum Reprod. 1998; 13 Suppl 3:161-74;discussion175-7. DOI: 10.1093/humrep/13.suppl_3.161. View

Boldt J, Tidswell N, Sayers A, Kilani R, Cline D . Human oocyte cryopreservation: 5-year experience with a sodium-depleted slow freezing method. Reprod Biomed Online. 2006; 13(1):96-100. DOI: 10.1016/s1472-6483(10)62021-4. View

Pickering S, Johnson M . The influence of cooling on the organization of the meiotic spindle of the mouse oocyte. Hum Reprod. 1987; 2(3):207-16. DOI: 10.1093/oxfordjournals.humrep.a136516. View

Bianchi V, Coticchio G, Fava L, Flamigni C, Borini A . Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration. Hum Reprod. 2005; 20(4):1078-83. DOI: 10.1093/humrep/deh736. View

10.

Kuwayama M, Vajta G, Ieda S, Kato O . Comparison of open and closed methods for vitrification of human embryos and the elimination of potential contamination. Reprod Biomed Online. 2006; 11(5):608-14. DOI: 10.1016/s1472-6483(10)61169-8. View

11.

MERYMAN H . Cryoprotective agents. Cryobiology. 1971; 8(2):173-83. DOI: 10.1016/0011-2240(71)90024-1. View

12.

Fabbri R, Porcu E, Marsella T, Rocchetta G, Venturoli S, Flamigni C . Human oocyte cryopreservation: new perspectives regarding oocyte survival. Hum Reprod. 2001; 16(3):411-6. DOI: 10.1093/humrep/16.3.411. View

13.

Webb M, Howlett S, Maro B . Parthenogenesis and cytoskeletal organization in ageing mouse eggs. J Embryol Exp Morphol. 1986; 95:131-45. View

14.

Aman R, Parks J . Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro-matured bovine oocytes. Biol Reprod. 1994; 50(1):103-10. DOI: 10.1095/biolreprod50.1.103. View

15.

Wennerholm W . Cryopreservation of embryos and oocytes: obstetric outcome and health in children. Hum Reprod. 2001; 15 Suppl 5:18-25. DOI: 10.1093/humrep/15.suppl_5.18. View

16.

Singh N, McCoy M, Tice R, Schneider E . A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988; 175(1):184-91. DOI: 10.1016/0014-4827(88)90265-0. View

17.

Magistrini M, Szollosi D . Effects of cold and of isopropyl-N-phenylcarbamate on the second meiotic spindle of mouse oocytes. Eur J Cell Biol. 1980; 22(2):699-707. View

18.

Fosas N, Marina F, Torres P, Jove I, MARTIN P, Perez N . The births of five Spanish babies from cryopreserved donated oocytes. Hum Reprod. 2003; 18(7):1417-21. DOI: 10.1093/humrep/deg297. View

19.

Porcu E, Fabbri R, Seracchioli R, Ciotti P, Magrini O, Flamigni C . Birth of a healthy female after intracytoplasmic sperm injection of cryopreserved human oocytes. Fertil Steril. 1997; 68(4):724-6. DOI: 10.1016/s0015-0282(97)00268-9. View

20.

Diedrich K, Al-Hasani S, Van der Ven H, Krebs D . Successful in vitro fertilization of frozen-thawed rabbit and human oocytes. Ann N Y Acad Sci. 1988; 541:562-70. DOI: 10.1111/j.1749-6632.1988.tb22292.x. View