Decreased Transport of P-aminohippurate in Renal Basolateral Membranes Isolated from Rats with Acute Renal Failure

Overview

Journal Pharm Res

Specialties Pharmacology
Pharmacy

Date 1989 Nov 1

PMID 2594687

Authors

K Inui

M Takano

H Maegawa

M Kato

R Hori

Affiliations

Soon will be listed here.

Abstract

Transport of D-glucose, p-aminohippurate (organic anion), and tetraethylammonium (organic cation) was studied in the renal basolateral membrane vesicles isolated from rats with acute renal failure (ARF). ARF was induced by a single injection of uranyl nitrate. Carrier-mediated transport of p-aminohippurate, estimated under anion-anion exchange condition, was significantly decreased in basolateral membrane vesicles isolated from ARF rats. In contrast, there were no significant differences in D-glucose and tetraethylammonium uptake between normal and ARF rats. When normal basolateral membrane vesicles were incubated in vitro with uranyl nitrate, no significant inhibition in p-aminohippurate uptake was observed. These results suggest that organic anion transport is decreased in renal basolateral membranes from ARF rats, and this transport dysfunction cannot be explained by the direct interaction of uranyl nitrate with the organic anion carrier.

References

Stein J, Lifschitz M, Barnes L . Current concepts on the pathophysiology of acute renal failure. Am J Physiol. 1978; 234(3):F171-81. DOI: 10.1152/ajprenal.1978.234.3.F171. View

Hori R, Takano M, Okano T, Kitazawa S, Inui K . Mechanisms of p-aminohippurate transport by brush-border and basolateral membrane vesicles isolated from rat kidney cortex. Biochim Biophys Acta. 1982; 692(1):97-100. DOI: 10.1016/0005-2736(82)90506-5. View

Murer H, Gmaj P . Transport studies in plasma membrane vesicles isolated from renal cortex. Kidney Int. 1986; 30(2):171-86. DOI: 10.1038/ki.1986.169. View

Takano M, Inui K, Okano T, Hori R . Cimetidine transport in rat renal brush border and basolateral membrane vesicles. Life Sci. 1985; 37(17):1579-85. DOI: 10.1016/0024-3205(85)90476-x. View

Holohan P, Ross C . Mechanisms of organic cation transport in kidney plasma membrane vesicles: 2. delta pH studies. J Pharmacol Exp Ther. 1981; 216(2):294-8. View

Inui K, Okano T, Takano M, Kitazawa S, Hori R . A simple method for the isolation of basolateral plasma membrane vesicles from rat kidney cortex. Enzyme activities and some properties of glucose transport. Biochim Biophys Acta. 1981; 647(1):150-4. DOI: 10.1016/0005-2736(81)90303-5. View

Shimada H, Moewes B, Burckhardt G . Indirect coupling to Na+ of p-aminohippuric acid uptake into rat renal basolateral membrane vesicles. Am J Physiol. 1987; 253(5 Pt 2):F795-801. DOI: 10.1152/ajprenal.1987.253.5.F795. View

Bennett W, Plamp 3rd C, Parker R, Gilbert D, Houghton D, Porter G . Alterations in organic ion transport induced by gentamicin nephrotoxicity in the rat. J Lab Clin Med. 1980; 95(1):32-9. View

Pritchard J . Luminal and peritubular steps in renal transport of p-aminohippurate. Biochim Biophys Acta. 1987; 906(2):295-308. DOI: 10.1016/0304-4157(87)90015-3. View

10.

Kasahara M, Inui K, Takano M, Hori R . Distinction of three types of D-glucose transport systems in animal cells. Biochem Biophys Res Commun. 1985; 132(2):490-6. DOI: 10.1016/0006-291x(85)91160-x. View

11.

Inui K, Takano M, Okano T, Hori R . Role of chloride on carrier-mediated transport of p-aminohippurate in rat renal basolateral membrane vesicles. Biochim Biophys Acta. 1986; 855(3):425-8. DOI: 10.1016/0005-2736(86)90088-x. View

12.

Kamiya A, Okumura K, Hori R . Quantitative investigation of renal handling of drugs in dogs with renal insufficiency. J Pharm Sci. 1984; 73(7):892-6. DOI: 10.1002/jps.2600730708. View

13.

Hirsch G . Differential effects of nephrotoxic agents on renal organic ion transport and metabolism. J Pharmacol Exp Ther. 1973; 186(3):593-9. View

14.

Pritchard J . Coupled transport of p-aminohippurate by rat kidney basolateral membrane vesicles. Am J Physiol. 1988; 255(4 Pt 2):F597-604. DOI: 10.1152/ajprenal.1988.255.4.F597. View

15.

Berner W, KINNE R . Transport of p-aminohippuric acid by plasma membrane vesicles isolated from rat kidney cortex. Pflugers Arch. 1976; 361(3):269-77. DOI: 10.1007/BF00587292. View

16.

Maegawa H, Kato M, Inui K, Hori R . pH sensitivity of H+/organic cation antiport system in rat renal brush-border membranes. J Biol Chem. 1988; 263(23):11150-4. View

17.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

18.

Hori R, Takano M, Okano T, Inui K . Transport of p-aminohippurate, tetraethylammonium and D-glucose in renal brush border membranes from rats with acute renal failure. J Pharmacol Exp Ther. 1985; 233(3):776-81. View

19.

Kasher J, Holohan P, Ross C . Na+ gradient-dependent p-aminohippurate (PAH) transport in rat basolateral membrane vesicles. J Pharmacol Exp Ther. 1983; 227(1):122-9. View

20.

Giacomini K, Roberts S, Levy G . Evaluation of methods for producing renal dysfunction in rats. J Pharm Sci. 1981; 70(2):117-21. DOI: 10.1002/jps.2600700202. View